Description
The cytokine that preserves the stem-cell-memory phenotype.
ISOkine® Interleukin-15 (IL-15) Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 114 amino acids and an N-terminal 17 amino acid histidine tag for a total length of 131 amino acids and has a predicted molecular mass of 15.04 kDa.
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Applications
- CAR-T & TIL Expansion: Preserves TSCM phenotype during long culture.
- NK Cell Proliferation & Survival: Supports expansion and cytotoxic function.
- Memory-like NK Induction: Essential in IL-12/IL-15/IL-18 protocols.
- iPSC-derived Immune Cells: Enables efficient expansion of NK and CAR-NK cells.
- NKT & CAR-NKT Differentiation: From hematopoietic progenitor cells.
- Defined Media Formulation: Ideal for serum-free, chemically-defined systems.
Summary
Interleukin-15 (IL-15) is a cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems. Stimulates the proliferation of natural killer cells, T-cells, and B-cells and promotes the secretion of several cytokines. In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection. Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits.
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
2xPBS pH 7.2, sterile filtered through 0.22 µm filter
Purity
≥ 90% by SDS-PAGE gel analysis.
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human IL-15 is assayed by measuring its dose-dependent effect on the proliferation of CTLL-2 cells. The ED50 for this effect using IL-15 is typically ≤2 ng/ml. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Endotoxin level is < 0.01 EU per μg of ISOkine® product by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Synonyms
Interleukin 15, IL15, UniProtKB# P40933.
Documents
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Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.