Description
The survival cytokine in every memory-enriched T-cell expansion protocol.
ISOkine® Interleukin-7 (IL-7) Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 152 amino acids and an N-terminal 17 amino acid histidine tag for a total length of 169 amino acids and has a predicted molecular mass of 19.7 kDa.
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Applications
- Lymphocyte Development & Expansion: Supports T-cell and B-cell proliferation and survival.
- Immune Response & Immunotherapy Research: Enhances immune function and anti-tumor activity.
- Stem Cell Research & Regenerative Medicine: Promotes stem cell maintenance and differentiation.
- Tissue Repair & Wound Healing: Supports tissue regeneration and recovery.
- Defined Media Formulation: Ideal for serum-free, chemically-defined systems.
Summary
Interleukin-7 (IL-7) is a hematopoietic cytokine that plays an essential role in the development, expansion, and survival of T-cells (naive and memory) and B-cells. Thereby helps with regulating the number of mature lymphocytes and maintaining lymphoid homeostasis by interaction with receptors composed of IL7RA and gamma/CSF2RG and activating various kinases (incl. JAK1 and JAK3).
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
2xPBS pH 7.2, sterile filtered through 0.22 µm filter
Purity
≥ 95% by SDS-PAGE gel analysis.
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human IL-7 is assayed by measuring its dose-dependent effect on the proliferation of 2E8 cells. The ED50 for this effect using IL-7 is typically ≤2 ng/ml. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Endotoxin level is < 0.01 EU per μg of ISOkine® product by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Synonyms
Interleukin 7, IL7, UniProtKB# P13232.
Documents
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Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.