ISOkine® TNF-alpha Human, Animal-Free Recombinant Protein, cat. no. IK2600, manufactured by ORF Genetics and distributed by Ilex Life Sciences.

ISOkine® TNF-alpha Human, Animal-Free Recombinant Protein

IK2600-0010
$69.00
$69.00
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Description

ISOkine® TNF-alpha Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 157 amino acids and an N-terminal 16 amino acid histidine tag for a total length of 173 amino acids and has a predicted molecular mass of 18.2 kDa.

ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.

Summary

Tumor necrosis factor alpha (TNF-alpha), a member of the TNF ligand superfamily, is among others, secreted by macrophages, activated monocytes, neutrophils, T-cells, B-cells, and natural killer cells stimulated by bacterial lipopolysaccharides. TNF-alpha shows approximately 30% homology with TNF-beta. TNF-alpha plays an important role in the regulation of immune cells. TNF-alpha has many biological activities, including a cytotoxic effect to various tumor cells, causes inflammation, cellular proliferation and plays a critical role in mediating resistance to bacterial infections. Human and murine TNF-alpha show approximately 79% homology and human TNF-alpha is active on murine cells with a slightly reduced specific activity.

Source

Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.

Manufacturer: ORF Genetics

Country of Origin: Iceland

Formulation

2xPBS pH 7.2, sterile filtered through 0.22 µm filter

Purity

> 95% by SDS-PAGE gel analysis.

Reconstitution

Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).

Stability

The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Biological Activity

Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human TNF-alpha is assayed by measuring its dose-dependent effect on the proliferation of actinomycin-D sensitized L929 cells. The ED50 for this effect using TNF-alpha is typically <0.4 ng/ml. Optimal concentration should be determined for specific applications and cell lines.

Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a&nbsp;commercial source. The bioactivity of ISOkine® recombinant human TNF-alpha is assayed by measuring its&nbsp;dose-dependent effect on the proliferation of actinomycin-D sensitized L929 cells. The ED50 for this effect using&nbsp;TNF-alpha is typically &lt;0.4 ng/ml. Optimal concentration should be determined for specific applications and&nbsp;cell lines.

Endotoxin Level

Endotoxin level is < 0.01 EU per μg of ISOkine® product by LAL method.

Pyrogen Free

Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.

Usage

This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

Synonyms

Human Tumor Necrosis Factor alpha, Human TNF-alpha, TNF alpha, TNF-a, TNF-α, TNF α, Tumor Necrosis Factor-α, TNF, TNFa, Cachectin, Tumor necrosis factor ligand superfamily member 2, TNFSF2, UniProtKB# P01375.

Documents

Product References

  • Cuomo, Francesca et al. “HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring.” Frontiers in cell and developmental biology vol. 9 740203. 12 Jan. 2022, doi:10.3389/fcell.2021.740203
  • Cuomo, Francesca et al. “Pro-inflammatory cytokines activate hypoxia-inducible factor 3α via epigenetic changes in mesenchymal stromal/stem cells.” Scientific reports vol. 8,1 5842. 11 Apr. 2018, doi:10.1038/s41598-018-24221-5
  • Lampronti, Ilaria et al. “Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells.” Mediators of inflammation vol. 2017 (2017): 2389487. doi:10.1155/2017/2389487
  • Kerr, Markus C et al. “Laser-mediated rupture of chlamydial inclusions triggers pathogen egress and host cell necrosis.” Nature communications vol. 8 14729. 10 Mar. 2017, doi:10.1038/ncomms14729

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Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.