Description
Our bioactive Tumor Necrosis Factor-alpha (TNF-alpha) Human, E. coli Recombinant Protein is a single, non-glycosylated polypeptide chain that contains 158 amino acids (157 a.a. of the mature human TNF-alpha and an N-terminal methionine) and has a molecular mass of 17.5 kDa. The TNF-alpha is purified by proprietary chromatographic techniques.
Summary
Tumor necrosis factor-alpha (TNF-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF-alpha is mainly secreted by macrophages. TNF-alpha is involved in apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis, and viral replication. TNF-alpha is also involved in lipid metabolism and coagulation. TNF-alpha's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF-alpha have been implicated in a variety of human diseases such as autoimmune diseases, insulin resistance, and cancer. Recombinant TNF-alpha is used extensively in biomedical laboratory research, particularly for studying its effects on cell signaling and immune response.
Other Names
Human Tumor Necrosis Factor alpha, Human TNF-alpha, TNF alpha, TNF-a, TNF-α, TNF α, Tumor Necrosis Factor-α, , TNF, TNFa, Cachectin, Tumor necrosis factor ligand superfamily member 2, TNFSF2, UniProtKB# P01375.
Source
Expressed in E. coli.
Formulation
Sterile filtered white lyophilized (freeze-dried) powder. The TNF-alpha was lyophilized from a concentrated (1 mg/ml) solution containing 20 mM PB, pH-7.2, and 100 mM NaCl.
Amino Acid Sequence
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL.
Purity
Greater than 95.0% as determined by:
- Analysis by RP-HPLC.
- Analysis by SDS-PAGE.
Biological Activity
The Specific Activity is > 5.0×107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Reconstitution
It is recommended to reconstitute the lyophilized TNF-alpha in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized TNF-alpha, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, TNF-alpha should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.