Neuropan-27 Supplement (50x), Defined Serum Substitute for Serum-Free Culture of CNS Neuronal Cells

Introduction

Neuropan-27 is provided as a 50x concentrated serum substitute designed for the long-term growth and survival of hippocampal and other neuronal cells of the Central Nervous System (CNS). By supplementing Neuropan Basal Medium with Neuropan-27 and 0.5 mM L-Glutamine, excellent long-term viability of rat embryonic hippocampal neurons has been achieved after several weeks in culture. Glial cell growth is reduced to less than 0.1% for a largely pure neuronal cell culture.

When using Neuropan-27 as a supplement to the Neuropan Basal Medium, we suggest to add 25 µM (2.7 µg/ml) L-glutamate to the medium for initial plating of primary hippocampal neurons. Medium changes after day 4 should be done without glutamate. When culturing neuroblastomas, glutamate should be included for both plating and subsequent culturing of cells. Improved long-term survival of hippocampal neurons may be obtained by the addition of beta-mercaptoethanol at 15-30 µM.

Application

When used as a supplement to Neuropan Basal Medium, Neuropan-27 has been demonstrated to give optimal growth and long-term survival of rat embryonic hippocampal neurons, and growth and survival of neurons from embryonic rat striatum, substantia nigra, septum, and cortex, and neonatal rat cerebellum and dentate gyrus. The combination of Neuropan Basal Medium with Neuropan-27 Supplement is also effective for the growth of tumor cell lines of neuronal origin. With additional supplementation with FGF-2, a combination of Neuropan Basal Medium with Neuropan-27 has been shown to support the growth of postnatal and adult rat hippocampal and cortical neurons. Neuropan-27 Supplement has been demonstrated to allow the expansion of EGF-responsive precursor cells from embryonic rat striatum and mesencephalon. Neuropan 27 in conjunction with Neuropan Basal Medium supports the growth of nearly pure populations of neural cells without the need of an astrocyte feeder layer. Neuropan-27 contains a cocktail of antioxidants to reduce reactive oxygen damage.

Advantages

In addition to low density growth of fetal hippocampal neurons, the combination of Neuropan-27 and Neuropan Basal Medium has been shown to support the growth of neurons from embryonic rat striatum, substantia nigra, septum, cortex and neonatal dentate gyrus and cerebellum. The combination of Neuropan-27 with Neuropan Basal Medium has been demonstrated to support the growth of postnatal and adult rat hippocampal and cortical neurons. In addition, a combination of Neuropan-27 with a DMEM/F12 mixture has been demonstrated to support the expansion of EGF-responsive precursor cells from rat embryonic striatum and mesencephalon.

Specifications

Neuropan-27 Supplement (50x), Cat. No. P07-07210, P07-07200.

Composition

Neuropan-27 Supplement is a serum substitute of defined composition containing albumin, insulin, transferrin, lipids, and hormones. It is used as a supplement to Neuropan Basal Medium to support growth and survival of neuronal cells from the CNS.

Documents

• Product Datasheet (PDS)
• Material Safety Data Sheet (MSDS)

Product References

• Pissas, Karolos-Philippos et al. “Functional expression of the proton sensors ASIC1a, TMEM206, and OGR1 together with BK_Ca channels is associated with cell volume changes and cell death under strongly acidic conditions in DAOY medulloblastoma cells.” Pflugers Archiv : European journal of physiology vol. 476,6 (2024): 923-937. doi:10.1007/s00424-024-02964-7
• Cortés Franco, Klaus-Daniel et al. “Aggressive migration in acidic pH of a glioblastoma cancer stem cell line in vitro is independent of ASIC and K_Ca3.1 ion channels, but involves phosphoinositide 3-kinase.” Pflugers Archiv : European journal of physiology vol. 475,3 (2023): 405-416. doi:10.1007/s00424-022-02781-w

Related Products

• Neuropan Basal Medium
• Neuropan-2 Supplement

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