Description
PAN-Biotech (10x) Trypsin 2.5% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in a 10x concentrated PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture.
Suitability
Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application.
Specifications
Cat. No. P10-022100
| Ca/Mg Content | Without Ca and Mg |
|
Concentration |
10x concentrated |
|
Liquid/Powder |
Liquid |
| Phenol Red | Without phenol red |
| Product Category | Enzymes for cell dissociation |
| Product Type | Trypsin |
| Size | 100 ml |
| Sterile | Yes |
| Storage Temperature | -20°C |
| Trypsin Concentration | 2.50% trypsin |
| In Solution | In PBS |
| Contaminants | Tested negative for mycoplasma and PPV. |
| Manufacturer | PAN-Biotech GmbH |
| Country of Origin | Germany |
Application Note
Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.
Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.
Resources
Product References
- Geladaris, Anastasia et al. “BTK inhibition limits microglia-perpetuated CNS inflammation and promotes myelin repair.” Acta neuropathologica vol. 147,1 75. 24 Apr. 2024, doi:10.1007/s00401-024-02730-0
- Charifou, Elsa et al. “A Robust Mammary Organoid System to Model Lactation and Involution-like Processes.” Bio-protocol vol. 11,8 e3996. 20 Apr. 2021, doi:10.21769/BioProtoc.3996
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