PAN-Biotech (10x) Trypsin 2.5 % in PBS, w/o: Ca and Mg, 100 ml bottle, cat. no. P10-022100, distributed by Ilex Life Sciences LLC

PAN-Biotech (10x) Trypsin 2.5% in PBS, w/o: Ca and Mg

P10-022100
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Description

PAN-Biotech (10x) Trypsin 2.5% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in a 10x concentrated PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture.

    Suitability

    Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application.

    Specifications

    Cat. No. P10-022100

    Ca/Mg Content Without Ca and Mg
    Concentration
    10x concentrated
    Liquid/Powder
    Liquid
    Phenol Red Without phenol red
    Product Category Enzymes for cell dissociation
    Product Type Trypsin
    Size 100 ml
    Sterile Yes
    Storage Temperature -20°C
    Trypsin Concentration 2.50% trypsin
    In Solution In PBS
    Contaminants Tested negative for mycoplasma and PPV.
    Manufacturer PAN-Biotech GmbH
    Country of Origin Germany

    Application Note

    Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.

    Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.

    Resources

    Product References

    • Geladaris, Anastasia et al. “BTK inhibition limits microglia-perpetuated CNS inflammation and promotes myelin repair.” Acta neuropathologica vol. 147,1 75. 24 Apr. 2024, doi:10.1007/s00401-024-02730-0
    • Charifou, Elsa et al. “A Robust Mammary Organoid System to Model Lactation and Involution-like Processes.” Bio-protocol vol. 11,8 e3996. 20 Apr. 2021, doi:10.21769/BioProtoc.3996

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      Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.