Description
PAN-Biotech Trypsin Inhibitor III is a sterile solution of purified proteins to inhibit trypsin proteolytic activity. It does not contain any animal or human components.
Supplied as 1 mg/ml in PBS solution.
Composition
Trypsin Inhibitor III solution contains natural protease inhibitor proteins. The biological activity is depending on trypsin concentration.
Suitability
For inhibiting trypsin activity in serum-free culture of adherent cells. Trypsin is used for dissociation of adhesion-dependent cells. Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells.
Inhibits trypsin activity; chymotrypsin and other proteolytic enzymes are inhibited to a lesser extent.
Trypsin Inhibitor III is cell culture tested and is appropriate for use in cell culture applications.
Specifications
Cat. No. P10-034105, P10-034100
|
Liquid/Powder: |
Liquid |
|
Product Category: |
Enzymes for cell dissociation |
|
Product Type: |
Trypsin inhibitor |
| Size: | Various |
|
Sterile: |
Yes |
| Storage Temperature: | -20°C |
| In Solution: | In PBS |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Application Note
Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.
Storage conditions & stability
Stability: 2 years from date of production
NOTE: Repeated freeze and thaw cycles will reduce activity and should be avoided. The product is stable for up to 2 years at recommended storage conditions. Thawed Trypsin Inhibitor III can be stored at 2-8°C for up to 1 month.
Resources
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Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.