Description
PAN-Biotech Trypsin Inhibitor III is a sterile solution of purified proteins to inhibit trypsin proteolytic activity. It does not contain any animal or human components.
Supplied as 1 mg/ml in PBS solution.
Composition
Trypsin Inhibitor III solution contains natural protease inhibitor proteins. The biological activity is depending on trypsin concentration.
Suitability
For inhibiting trypsin activity in serum-free culture of adherent cells. Trypsin is used for dissociation of adhesion-dependent cells. Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells.
Inhibits trypsin activity; Chymotrypsin and other proteolytic enzymes are inhibited to a lesser extent.
Trypsin Inhibitor III is cell culture tested and is appropriate for use in cell culture applications.
Application Note
Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.
Storage conditions & stability
Stability: 2 years from date of production
Repeated freeze and thaw cycles will reduce activity and should be avoided. The product is stable for up to 2 years at recommended storage conditions. Thawed Trypsin Inhibitor III can be stored at 2-8°C for up to 1 month.
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Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.