Description
PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water.
Composition
PAN-Biotech Gelatine Solution 2% in DPBS, w/o: Ca, Mg
Application
Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media.
Specifications
Cat. No. P06-25200
| Liquid / Powder: | liquid |
|---|---|
| Product Category: | Attachment Factors |
| Product Type: | Gelatin Solution |
| Size: | various |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Coating Procedure
Coating of cell culture surfaces using PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg:
- NOTE: Optimal conditions for attachment must be determined for each cell line and application.
- Allow gelatin solution to completely dissolve at 37°C.
- Coat culture surface with 5-10 µl gelatin solution per cm^2. (0.1 - 0.2 mg per cm^2 gelatin).
- Allow surface to dry for at least 2 hours before introducing cells and medium.
Documents
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Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.