PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg, 500 ml bottle, cell culture attachment factor, cat. no. P06-20410, distributed by Ilex Life Sciences.

PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg

P06-20410
$45.00
$45.00
$53.00
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Description

PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water.

Composition

PAN-Biotech Gelatine Solution 0.1% in DPBS, w/o: Ca, Mg

Application

Applications using gelatin include coating cell culture plates, flasks, or dishes to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. 

Specifications

Cat. No. P06-20410

Liquid / Powder: liquid
Product Category: Attachment Factors
Product Type: Gelatin Solution
Size: 500 ml bottles
Sterile: Yes
Ca & Mg: Without Ca and Mg
Storage Temperature: +2°C - +8°C
Manufacturer: PAN-Biotech GmbH
Country of Origin: Germany

Coating Procedure

Coating of cell culture surfaces using PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg:

  • NOTE: Optimal conditions for attachment must be determined for each cell line and application.
  • Allow gelatin solution to completely dissolve at 37°C.
  • Coat culture surface with 100-200 µl gelatin solution per cm^2. (0.1 - 0.2 mg per cm^2 gelatin) and incubate at 37°C for at least 30 minutes.
  • Remove excess gelatin solution aseptically and allow surface to dry for at least 2 hours before introducing cells and medium.

Documents

Product References

  • Grzonka, Marta, and Hisham Bazzi. “Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells.” eLife vol. 13 e94694. 26 Feb. 2024, doi:10.7554/eLife.94694
  • Gazorpak, Mahshid et al. “Harnessing PROTAC technology to combat stress hormone receptor activation.” Nature communications vol. 14,1 8177. 9 Dec. 2023, doi:10.1038/s41467-023-44031-2
  • Yayli, Gizem et al. “ATAC and SAGA co-activator complexes utilize co-translational assembly, but their cellular localization properties and functions are distinct.” Cell reports vol. 42,9 (2023): 113099. doi:10.1016/j.celrep.2023.113099
  • Fischer, Veronique et al. “SUPT3H-less SAGA coactivator can assemble and function without significantly perturbing RNA polymerase II transcription in mammalian cells.” Nucleic acids research vol. 50,14 (2022): 7972-7990. doi:10.1093/nar/gkac637
  • Scheer, Elisabeth et al. “TAF8 regions important for TFIID lobe B assembly or for TAF2 interactions are required for embryonic stem cell survival.” The Journal of biological chemistry vol. 297,5 (2021): 101288. doi:10.1016/j.jbc.2021.101288
  • Adihou, Hélène et al. “A protein tertiary structure mimetic modulator of the Hippo signalling pathway.” Nature communications vol. 11,1 5425. 27 Oct. 2020, doi:10.1038/s41467-020-19224-8

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Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.