Description
PAN-Biotech Trypsin 0.05% / EDTA 0.02% in PBS, w/o: Ca and Mg, w: Phenol Red is a porcine pancreas-derived enzyme plus EDTA in a PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture.
Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts.
Suitability
Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application.
Specifications
Cat. No. P10-0231SP, P10-0235SP
Ca/Mg Content: | Without Ca and Mg |
Concentration |
1x |
Trypsin Concentration | 0.05% Trypsin |
EDTA Concentration |
0.02% EDTA |
Liquid/Powder |
Liquid |
Phenol Red | With phenol red |
Product Category | Enzymes for cell dissociation |
Product Type | Trypsin-EDTA |
Size | Various |
Sterile | Yes |
Storage Temperature | -20°C |
In Solution | In PBS |
Contaminants | Tested negative for mycoplasma and PPV. |
Manufacturer | PAN-Biotech GmbH |
Country of Origin | Germany |
Application Note
Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.
Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.
Resources
- P10-0231SP Product Datasheet (PDS)
- P10-0235SP Product Datasheet (PDS)
- P10-0231SP Product Composition
- P10-0235SP Product Composition
- P10-0231SP Material Safety Data Sheet (MSDS)
- P10-0235SP Material Safety Data Sheet (MSDS)
Product References
- Weklak, Denice et al. “Insights from the Construction of Adenovirus-Based Vaccine Candidates against SARS-CoV-2: Expecting the Unexpected.” Viruses vol. 15,11 2155. 25 Oct. 2023, doi:10.3390/v15112155
- Reppert, Nikolas, and Thorsten Lang. “A conserved sequence in the small intracellular loop of tetraspanins forms an M-shaped inter-helix turn.” Scientific reports vol. 12,1 4494. 16 Mar. 2022, doi:10.1038/s41598-022-07243-y
- Finke, Jérôme et al. “HPV caught in the tetraspanin web?.” Medical microbiology and immunology vol. 209,4 (2020): 447-459. doi:10.1007/s00430-020-00683-1
- Finke, Jérôme et al. “Anatomy of a viral entry platform differentially functionalized by integrins α3 and α6.” Scientific reports vol. 10,1 5356. 24 Mar. 2020, doi:10.1038/s41598-020-62202-9
- Weber, Pascal et al. “Electrostatic anchoring precedes stable membrane attachment of SNAP25/SNAP23 to the plasma membrane.” eLife vol. 6 e19394. 27 Feb. 2017, doi:10.7554/eLife.19394
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