PAN-Biotech Trypsin 0.05% / EDTA 0.1% in PBS, w/o: Ca and Mg, 100 ml bottle, cat. no. P10-027100, distributed by Ilex Life Sciences LLC

PAN-Biotech Trypsin 0.05% / EDTA 0.1% in PBS, w/o: Ca and Mg

P10-027100
$35.00
$35.00
$41.00
Unavailable
Shipping calculated at checkout.

Description

PAN-Biotech Trypsin 0.05% / EDTA 0.1% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture.

Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts.

    Suitability

    Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application.

    Specifications

    Cat. No. P10-027100

    Ca/Mg Content: Without Ca and Mg
    Concentration
    		 1x
    Trypsin Concentration 0.05% Trypsin
    EDTA Concentration
    		 0.1% EDTA
    Liquid/Powder
    		 Liquid
    Phenol Red Without phenol red
    Product Category Enzymes for cell dissociation
    Product Type Trypsin-EDTA
    Size Various
    Sterile Yes
    Storage Temperature -20°C
    In Solution In PBS
    Contaminants Tested negative for mycoplasma and PPV.
    Manufacturer PAN-Biotech GmbH
    Country of Origin Germany

    Application Note

    Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.

    Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.

    Resources

    Product References

    • Jatho, Aline et al. “The HIFα-Stabilizing Drug Roxadustat Increases the Number of Renal Epo-Producing Sca-1+ Cells.” Cells vol. 11,4 753. 21 Feb. 2022, doi:10.3390/cells11040753

    Related Products

      Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.