PAN-Biotech Trypsin 0.25% / 1 mM EDTA 4 Na in PBS, w/o: Ca and Mg

Description

PAN-Biotech Trypsin 0.25% / 1 mM EDTA 4 Na in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA·4Na in a PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture.

Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts.

Suitability

Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application.

Specifications

Cat. No. P10-028100

Application Note

Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.

Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.

Resources

• P10-028100 Product Composition
• P10-028100 Material Safety Data Sheet (MSDS)

Related Products

• Enzymes for cell dissociation
  
• Bovine Trypsin, recombinant, expressed in corn, lyophilized powder

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