Description
Density gradient centrifugation media to easily isolate granulocytes from red blood cells!
In many cases the isolation of cells is the first step for gene expression studies or in diagnostic procedures. Besides biological separation techniques physical separation methods are most commonly used. These methods use physical differences such as size and weight of the particles to be separated. For this purpose so-called separating solutions (also known as centrifugation media or density gradient media) are used.
Pancoll separation solutions from PAN-Biotech contain a polysaccharide with a molecular weight of 400,000 daltons. This hydrophilic polymer allows production of aqueous solutions for cell separation with a density of up to 1.119 g/ml. PAN-Biotech offers a variety of ready-to-use products with a density of 1.063 g/ml up to 1.119 g/ml for a very wide range of cell separation applications.
As the densities of blood fractions and the osmolality are different among species these parameters are optimized in Pancoll rat, Pancoll mouse and Pancoll animal.
Specifications
To isolate granulocytes we recommend to combine Pancoll Human 1.077 and Pancoll Granulocytes 1.119. Due to aggregation of erythrocytes induced by the Pancoll media granulocytes can be isolated from the red blood cells.
- Pancoll Human for Granulocytes: Cell Separation Medium, Density: 1.119 g/ml
| Density: | 1.119 g/mL |
|---|---|
| EDTA: | Without EDTA |
| Endotoxin Content: | Endotoxin content unspecified |
| HEPES: | Without HEPES |
| Liquid / Powder: | Liquid |
| Product Category: | Cell Separation Media |
| Product Type: | Pancoll Cell Separation Media |
| Size | Various |
| Sterile: | Yes |
| Storage Temperature: | +2°C - RT |
Method of Separation
Pancoll is available in different densities of the separation solution (e.g. 1.068 g/ml, 1.077 g/ml). The density is the key parameter to choose the appropriate solution for the target fraction (see table 1).
Table 1: Density of cell fractions of human blood
| Cell Fraction | Density (g/ml) |
| Thrombocytes | 1.040 - 1.060 |
| Monocytes | 1.059 - 1.068 |
| Lymphocytes | 1.066 - 1.077 |
| Basophiles | 1.075 - 1.081 |
| Neutrophiles | 1.080 - 1.099 |
| Eosinophiles | 1.088 - 1.096 |
| Erythrocytes | 1.090 - 1.110 |
Reference 1. Separation by Cell Size and Cell Density, Immunology (The Experimenter Series), Werner Luttmann, Kai Bratke, et al., Academic Press Publications 2006, ISBN-13 : 978-0120885442
Typical Results
| Lymphocytes | 60 ± 20 % | yield of Lymphocytes from original blood samples |
| 95 ± 5 % | of the Lymphocyte fraction are mononuclear Leukocytes | |
| > 90 % | live cells (trypan blue-exclusion) | |
| Other cells | 3 ± 2 % | Granulocytes |
| 5 ± 2 % | Erythrocytes | |
| < 0.5 % | total number of platelets of the original blood sample |
Storage conditions & stability
When properly stored, density gradient separation solutions are stable for at least 36 months. The storage period starts with the manufacturing date.
Resources
- Product Datasheet (PDS)
- P04-60110: Material Safety Data Sheet (MSDS)
- P04-60150: Material Safety Data Sheet (MSDS)
- Pancoll Product References
Related Products
Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.