Introduction
PAN-Biotech Panserin 293A is a ready-to-use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in adherent culture. Serum-containing medium can be replaced directly.
Panserin 293A promotes the attachment of the cells and high cell growth rates, and has been used for the expression of recombinant proteins and the proliferation of AVVs and LVVs. The efficient serum-free cultivation can be supported by higher seeding densities.
NOTE: For serum-free cultivation of HEK293 cells in suspension culture, please see Panserin 293S.
Specifications
| Cell Type: | HEK cells |
|---|---|
| Culture Type: | Adherent Culture |
| Liquid / Powder: | liquid |
| Product Category: | Serum-Free Media |
| Glutamine: | With L-Glutamine |
| Size: | 100 ml or 500 ml bottles |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Composition
Based on DMEM, Panserin 293A contains additional trace elements, proteins, cholesterol, lipids, vitamins and hormones. The total content of proteins and animal-derived components is lower than 0.2% w/v.
Application
Panserin 293A is a particularly enriched medium optimized for the growth of HEK293 cells in adherent culture. HEK293 is frequently used for the expression of recombinant proteins and the proliferation of adenoviruses. Panserin 293A promotes a rapid attachment of the cells and high cell growth rates.
A switch from serum-containing medium to Panserin 293A is often possible without adaptation. For those clones that do not tolerate an immediate switch, we recommend a primary culture with serum containing medium and a stepwise reduction of serum towards a serum-free cultivation with Panserin 293A.
The efficient serum-free cultivation is supported by higher seeding densities. The viability of the cells is an important factor for a successful transfer into serum-free cultivation. The cells should be transferred in the logarithmic growth phase. According to our experience, the transfer within the logarithmic growth phase will have higher prospects of success. During the cell transfer it should assured that if trypsin is used for detachment, that the enzyme is completely washed out or is inactivated by trypsin inhibitors.
Documents
Product References
- Gerhards, Johanna et al. “Enzymatic vitamin A2 production enables red-shifted optogenetics.” Pflugers Archiv : European journal of physiology vol. 475,12 (2023): 1409-1419. doi:10.1007/s00424-023-02880-2
- Ostendorf, Thomas et al. “Immune Sensing of Synthetic, Bacterial, and Protozoan RNA by Toll-like Receptor 8 Requires Coordinated Processing by RNase T2 and RNase 2.” Immunity vol. 52,4 (2020): 591-605.e6. doi:10.1016/j.immuni.2020.03.009
- Burton, Bronwen R et al. “Variant proteins stimulate more IgM+ GC B-cells revealing a mechanism of cross-reactive recognition by antibody memory.” eLife vol. 7 e26832. 1 May. 2018, doi:10.7554/eLife.26832
- Hanauer, Jan Rh et al. “Enhanced lysis by bispecific oncolytic measles viruses simultaneously using HER2/neu or EpCAM as target receptors.” Molecular therapy oncolytics vol. 3 16003. 24 Feb. 2016, doi:10.1038/mto.2016.3
- Friedel, Thorsten et al. “Receptor-targeted lentiviral vectors are exceptionally sensitive toward the biophysical properties of the displayed single-chain Fv.” Protein engineering, design & selection : PEDS vol. 28,4 (2015): 93-106. doi:10.1093/protein/gzv005
- Dreyer, Anita M et al. “Passive immunoprotection of Plasmodium falciparum-infected mice designates the CyRPA as candidate malaria vaccine antigen.” Journal of immunology (Baltimore, Md. : 1950) vol. 188,12 (2012): 6225-37. doi:10.4049/jimmunol.1103177
Related Products
- Panexin NTA: Fully-Defined Serum Substitute for Adherent Cells
- Panserin 293S: Serum-free medium for HEK293 cells in suspension culture
- Cell Dissociation Enzymes/Reagents
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