PAN-Biotech Panserin 413: Serum-Free Medium for Lymphocytes, T Cells, and Hybridoma Cells, w: L-Glutamine, modified, 500 ml cell culture media
PAN-Biotech Panserin 413: Serum-Free Medium for Lymphocytes, T Cells, and Hybridoma Cells, w: L-Glutamine, modified, 1000 ml cell culture media
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Panserin 413: Serum-Free Medium for Lymphocytes, T Cells, and Hybridoma Cells, w: L-Glutamine, modified

P04-71413S
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$135.00
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Introduction

PAN-Biotech Panserin 413 is a ready-to-use, serum-free, low protein (<0.1% w/v) and low animal-derived components (< 0.6%) medium for the serum-free cultivation and proliferation of lymphocytes from whole blood. The medium can be used for the enrichment of T cells , cytokine-induced killer (CIK) cells, lymphokine-activated killer (LAK) cells, and natural killers (NK) cells. It allows:

  • Expansion of T-Cells from PBMC / whole blood after stimulation (e.g. with PHA-L or anti-CD3).
  • Expansion of NK-, LAK- and CIK-cells after stimulation.
  • Long-term cultivation of immortalized T-cells.
    Cell Type: T cells, CIK cells, LAK cells, NK cells
    Culture Type: Suspension Culture
    Liquid / Powder: liquid
    Product Category: Serum-Free Media
    Glutamine With L-Glutamine
    Size: Various
    Sterile: Yes
    Storage Temperature: +2°C - +8°C

    Composition

    This defined formulation based on RPMI 1640 and DMEM/F12 is optimized with additional salts, trace elements, albumin, cholesterol, lipids and vitamins. The medium contains no hydrolysates, and only traces of animal-derived components (< 0.6% w/v) and proteins (< 0.1% w/v). IMPORTANT: Mitogens, such as PHA-L, or antibodies, such as CD3+, are necessary for the cell differentiation or the enrichment of T-cells from whole blood/PBMC. These reagents are not included.

    Application

    Panserin 413 modified is a ready-to-use medium optimized for the serum-free in vitro cultivation of human immune cells isolated from peripheral whole blood. The Panserin 413 modified formulation allows the efficient expansion of T-cells, CIK, LAK, and NK cells without the addition of bovine serum or (autologous) human serum.

    For isolation of PBMCs / lymphocytes from whole blood by density gradient centrifugation, we recommend using Pancoll Human: Cell Separating Solution, Density 1.077 g/ml.

      Storage conditions & stability

      Store at +2°C - +8°C.

      Documents

      Product References

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      • Boulos, Joelle C et al. “Repurposing of the ALK Inhibitor Crizotinib for Acute Leukemia and Multiple Myeloma Cells.” Pharmaceuticals (Basel, Switzerland) vol. 14,11 1126. 5 Nov. 2021, doi:10.3390/ph14111126
      • Özenver, Nadire et al. “Chemometric and Transcriptomic Profiling, Microtubule Disruption and Cell Death Induction by Secalonic Acid in Tumor Cells.” Molecules (Basel, Switzerland) vol. 25,14 3224. 15 Jul. 2020, doi:10.3390/molecules25143224
      • El-Daher, Marie-Therese et al. “Tetratricopeptide repeat domain 7A is a nuclear factor that modulates transcription and chromatin structure.” Cell discovery vol. 4 61. 13 Nov. 2018, doi:10.1038/s41421-018-0061-y
      • Özenver, Nadire et al. “Aloe-emodin as drug candidate for cancer therapy.” Oncotarget vol. 9,25 17770-17796. 3 Apr. 2018, doi:10.18632/oncotarget.24880
      • Saeed, Mohamed E M et al. “Molecular Determinants of Sensitivity or Resistance of Cancer Cells Toward Sanguinarine.” Frontiers in pharmacology vol. 9 136. 26 Feb. 2018, doi:10.3389/fphar.2018.00136

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