PAN-Biotech Phytohemagglutinin-L (PHA-L) from Phaseolus vulgaris, Lyophilized

Description

Phytohemagglutinin-L (PHA-L) is a plant lectin purified from Phaseolus vulgaris (red kidney bean).

Lectins are an important mitogen group. Lectins bind specifically to membrane-glycoproteins of different cells, including lymphocytes. The agglutinating effect leads to lymphocyte activation and proliferation.

Phytohemagglutinins have several binding sites to carbohydrates located in the cell membrane of the targeted cells. PHA-L recognizes complex oligosaccharides and binds to them leading to an agglutinating effect. Binding of the mitogen to the cell membrane seems to increase the molecular/information flow between the cell and its environment. Increased membrane permeability activates the synthesis of proteins and nucleic acids. The synthesis rate for messenger substances like lymphokines (e.g. IL-2) is also increased.

Composition

Phytohemagglutinin-L (PHA-L) purified from Phaseolus vulgaris (red kidney bean).

Application

Plant lectin suitable for stimulating lymphocyte proliferation and differentiation.

Reconstitute PHA-L with 2 ml deionized water (concentration PHA-L solution: 5 mg/ml). Further dilute with deionized water, DPBS, or cell culture media to final concentration. Filter sterilize the reconstituted solution with a 0.2 µm filter. It is recommended to make aliquots to avoid freeze and thaw cycles of the solution. NOTE: You may sterilize the reconstituted solution before diluting to final concentration. For further dilution, use sterile liquids and aseptic techniques.

Further instructions for using PHA-L for lymphocyte stimulation can be found in the datasheet for Panserin 413.

Specifications

Other names

Phaseolus Vulgaris Lectin L, PHA L subunit, Leucoagglutinating phytohemagglutinin, DLEC2, Leucoagglutinin, UniProtKB# P05087

Documents

• Product Data Sheet (PDS)

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