Description
Serum replacements for cell culture media address the scalability, performance, sustainability and ethical challenges of fetal bovine serum (FBS). However, developing effective alternatives has traditionally been costly and time-consuming. This delays new applications of cell culturing in getting from lab to market.
Multus Biotechnology Ltd. makes development of animal component-free (ACF) media cheaper and faster, delivering affordable, high-performance media for a wider variety of cells. Multus products are designed to help establish scalable processes for animal cell culturing.
Proliferum® P is a next-generation serum replacement specifically engineered for adipose-derived stem cells (ASCs). ASCs are multipotent cells that differentiate into fat, bone, and cartilage, making them valuable for food production, regenerative medicine, and therapeutics. Using Multus' proprietary media optimization platform and deep expertise in cellular growth requirements and ingredient functionality, Proliferum® P represents a breakthrough in both development speed and performance.
Proliferum® P represents the first commercially available animal component-free (ACF) formulation specifically designed for porcine ASCs, delivering performance that matches or exceeds fetal bovine serum (FBS) while maintaining critical stemness characteristics and adipogenic differentiation capacity. The cost-effective formulation offers a reliable, scalable, quality-controlled alternative with significant performance advantages over traditional FBS.
The Proliferum® P Panel of three different formulations is designed to support the growth of ASCs. Upon determining which of the three formulations works best for your cells, you may purchase that formulation on its own.
Specifications
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pH: |
6.5 - 8.5 |
| Osmolarity (mOsm/kg): | 290 - 320 |
| Bacteria Testing: | Negative |
| Mycoplasma Testing: | Negative |
| Fungal Testing: | Negative |
| Particulate Examination: | Negative |
| Cell Growth: | Pass |
| Storage: | -20°C, protect from light |
| Manufacturer: | Multus Biotechnology Ltd. |
| Country of Origin: | United Kingdom |
Performance
Figure 1: Average doubling times from 5 passages between 10% FBS and 10% (1X) Proliferum® P in DMEM/F12. The cell models tested are piADSCs (porcine immortalized adipocyte derived stem cells-genetically engineered) and psiADSCs (porcine spontaneously immortalized adipocyte derived stem cells).
Figure 2: Morphology of piADSCs (left column) and psiADSCs (right column) porcine cells after 5 passages in media supplemented with three different Proliferum P formulations (PP01, PP02, PP03), or 10% FBS.
Figure 3: Adipose differentiation test on piADSCs (left column) and psiADSCs (right
column) porcine cells after 3 passages in media supplemented with three different Proliferum P formulations (PP01, PP02, PP03), or 10% FBS.
Figure 4: Live (green) / dead (red) cell staining on piADSCs (left) and psiADSCs (right)
porcine cells after culture with Cytodex III microcarriers in media supplemented with Proliferum P formulation PP03 or 10% FBS.
Complete Media Preparation
For cell culturing dilute in basal media (DMEM/F12 recommended) to your desired concentration. We recommend 1-2X but suggest titration experiments to determine the optimal concentration for your cells. Ensure the complete media has reached 25°C prior to cell culturing and minimize light exposure.
|
1 L Proliferum P (1X) |
1 L Proliferum P (2X) |
|
|
Usage: |
Replaces 1 L of 10% FBS | Replaces 1 L of 20% FBS |
|
Proliferum P (10X): |
100 ml | 200 ml |
|
DMEM/F12: |
900 ml | 800 ml |
Adaptation
Switching directly from FBS to Proliferum® P is recommended (with an appropriate tissue culture coating). If cells exhibit poor viability and/or growth, we recommend the following adaptation protocol:
| Passage 1: | 50% FBS medium : 50% Proliferum® P medium |
| Passage 2: | 25% FBS medium : 75% Proliferum® P medium |
| Passage 3: | 0% FBS medium : 100% Proliferum® P medium |
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Other Names
FBS replacement, FBS substitute, serum substitute
Resources
Related Products
- Multus Food Grade DMEM/F12 Cell Culture Medium
- Proliferum® B Panel: Animal-Free Serum Substitute (developed with bovine fibroblasts)
- Serum Replacements
- DMEM/F12
Ilex Life Sciences LLC is an authorized distributor of Multus Biotechnology Limited.