Introduction
Traditional basal media formulations like DMEM/F12 have long been essential for cell culture but remain unsuitable for large-scale food production due to pharmaceutical-grade components and regulatory challenges. This creates significant barriers for companies in the cellular agriculture sector seeking to move from lab to commercial scale.
Multus Biotechnology has developed DMEM/F12-FG, a food-grade basal media specifically designed to address these challenges.
By leveraging AI and automation to identify functionally equivalent food-grade ingredients, the medium provides essential nutrients required for optimal cell growth while ensuring regulatory compliance. Formulated to food-grade specifications and manufactured in a FSSC 22000-certified facility, DMEM/F12-FG was developed in collaboration with global food and feed ingredient companies to ensure supply chain stability and scalability for confident advancement toward commercial production.
Quality Control & Specifications
| pH: | 7.0 - 7.5 |
|---|---|
| Osmolarity (mOsm/kg): | 290 - 340 |
| Bacterial Testing: | Negative |
| Fungal Testing: | Negative |
| Mycoplasma Testing: | Negative |
| Particulate Examination: | Negative |
| Cell Growth: | Pass |
| Bicarbonate: | With Sodium Bicarbonate (2438.0 mg/L) |
| Glucose: | With glucose (3151.00 mg/L) |
| Glutamine: | With L-Glutamine (365.0 mg/L) |
| HEPES: | Without HEPES |
| Liquid / Powder: | Liquid |
| Phenol Red: | Without Phenol Red |
| Product Category: | Cell Culture Media |
| Product Type: | DMEM/F12 |
| Size: | 500 ml or 1000 ml bottles |
| Sodium Pyruvate: | Without Sodium Pyruvate |
| Sterile Filtration: | 0.2 μm filtered |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | Multus Biotechnology Limited |
| Country of Origin: | United Kingdom |
Performance
Figure 1: C2C12 Growth in T25 Flasks over 5 Passages. Left panel shows doubling time comparison between cells cultured in Multus DMEM/F12-FG (blue) and pharma-grade DMEM/F12 (pink), p = 0.986 (n.s.). Right panel shows viability comparison between the same conditions, p = 0.145 (n.s.). Both media were supplemented with 1X Proliferum M serum.
Figure 2: C2C12 Growth Profile Over Time in 96 Well Plates Over 4 Passages. Comparison
between cells cultured in Multus DMEM/F12-FG (blue) and pharma-grade DMEM/F12
(pink) supplemented with 1X Proliferum M serum (n=5 per condition). No statistically
significant differences were observed between culture conditions
Figure 3: C2C12 cell morphology after 3 passages in different media. Left: Multus FG DMEM/F12; Right: PharmaGrade DMEM/F12. Both media supplemented with 1X Proliferum M serum. Phase contrast microscopy at 200 μm scale shows comparable cell morphology between conditions.
Documents
Related Products
- Proliferum® B Panel: Animal-Free Serum Substitute (developed with bovine fibroblasts)
- Proliferum® P Panel: Animal-Free Serum Substitute (developed with porcine adipose-derived stem cells)
Ilex Life Sciences LLC is an authorized distributor of Multus Biotechnology Limited.