Ilex Life Sciences bioactive lyophilized Fibroblast Growth Factor 1 (FGF-acidic) Human, E. coli Recombinant Protein

Fibroblast Growth Factor 1 (FGF-acidic) Human, E. coli Recombinant Protein

A32640010
Regular price
$125.00
Sale price
$125.00
Regular price
Available January 20th
Unit price
per 
Shipping calculated at checkout.

Description

Our bioactive Fibroblast Growth Factor 1 (FGF-acidic) Human, E. coli Recombinant Protein is a single, non-glycosylated, polypeptide chain containing 140 amino acids and has a molecular mass of approximately 15.8 kDa. The FGF-acidic is purified by proprietary chromatographic techniques.

Summary

FGF-acidic, a member of the fibroblast growth factor (FGF) family, is a non-glycosylated heparin binding growth factor encoded by the FGF1 gene. FGF-acidic plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and FGFR1 are essential for FGF1 signaling.

Other Names

Fibroblast Growth Factor-1, FGF1, FGF-1, FGF acidic, FGF-a, Acidic fibroblast growth factor, aFGF, Heparin-binding growth factor 1, HBGF-1.

Amino Acid Sequence

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Source

E. coli

Formulation

Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing PBS, pH 7.4.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity

The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is <0.5 ng/ml, corresponding to a specific activity of > 2,000,000 IU/mg.

Reconstitution

It is recommended to reconstitute the lyophilized FGF-acidic in sterile 18MΩ-cm H2O at 4 degrees Celsius at a concentration of 0.1 mg - 0.25 mg per 1 ml. Allow sample to sit for 5 min. at 4 degrees, spin to remove precipitant.

Shipping

At ambient temperature.  Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized FGF-acidic, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-acidic should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Usage

This product is intended for Laboratory Research Use Only. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

Resources

Want to learn more about FGF-acidic? We have compiled the links below which contain information that you may find interesting.