Description
Recombinant Human Albumin (rHA), expressed in rice, lyophilized powder is a non-glycosylated, polypeptide chain containing 585 amino acids and has a molecular mass of 67 kDa. The optimum concentration for recombinant albumin to be used in cell culture ranges between 0.5 g - 2 g per liter. The recombinant albumin is purified by proprietary chromatographic techniques.
Applications
Due to free hydrophobic regions albumins are used to solubilize lipids and stabilize other proteins such as growth factors in tissue culture applications. They are also used as blocking agents in Western blots or ELISA applications and as a protein standard. Albumin has a low molecular weight, is easily soluble in water and contains an excess of acidic amino acids.
- Cell and tissue culture media supplement
- Used as serum substitute in serum-free, xeno-free cell culture
- Medium component for cryopreservation and stem cell culture
- Stabilizer protein in biochemical preparations
- Reduces unspecific binding in molecular biology assays
Specifications
| Product Category: |
Media supplements |
| Product Type: |
HSA / BSA |
|
CAS Registry No.: |
70024-90-7 |
| Source: |
Expressed in Rice Grain plant (Oryza sativa) |
| Appearance: |
Sterile-filtered, white, lyophilized powder. |
| Formulation: | The rHA was lyophilized with sodium chloride. A 10% w/v solution when dissolved in water will contain 50mM NaCl. |
| Purity (SDS-PAGE): |
> 98% |
| Shipping Condition: | Shipped at ambient temperature. Upon receipt, store the product at the temperature recommended below. |
| Storage Temperature: | rHA, although stable at 4°C for 3 weeks, should be stored at -18°C. Please prevent freeze-thaw cycles. |
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Documents
Other Names
Recombinant Human Serum Albumin, Plant Recombinant HSA, rHSA, OSrHSA
Background
Albumins are monomeric proteins found in body fluids and tissues. Human serum albumin (HSA) is the most abundant protein in human blood plasma with a molecular weight of about 66.5 kDa. The major biological function of albumin is to regulate the colloidal osmotic blood pressure. In addition, albumin acts as carrier protein for hormones, fatty acids and other compounds, and buffers pH. The reference range for albumin concentration in serum is approximately 35-50 g/L.