Description
Our bioactive VEGF-165 Human, E. coli Recombinant Protein is a double, non-glycosylated, polypeptide chain that contains 165 amino acids and has a molecular mass of 38.2 kDa. The VEGF-165 is purified by proprietary chromatographic techniques.
Summary
Vascular endothelial growth factor (VEGF), also known as VEGF-A, is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells ).
VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.
Alternately spliced VEGF isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) are expressed in humans, with VEGF-165 being known to be the most abundant and potent isoform.
Other Names
VEGF-A, VEGFA, Vascular endothelial growth factor (VEGF), Vascular Endothelial Growth Factor 165, VEGF165, VEGF 165, Vascular permeability factor (VPF), UniProtKB# P15692
Source
Expressed in E. coli.
Formulation
Amino Acid Sequence
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.
Purity
Purity greater than 95.0% as determined by:
- Analysis by RP-HPLC.
- Analysis by SDS-PAGE.
Protein Content
VEGF-165 protein quantitation was carried out by two independent methods:
- UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
- Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard.
Biological Activity
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 3.7-5.6 ng/ml, corresponding to a Specific Activity of 178,570-270,270 IU/mg.
Reconstitution
It is recommended to reconstitute the lyophilized VEGF-165 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
Ships at ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized VEGF-165, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, VEGF-165 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.