Description
ISOkine® VEGF-165 Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 165 amino acids and an N-terminal 6 amino acid histidine tag for a total length of 171 amino acids and has a predicted molecular mass of 20.0 kDa.
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Summary
VEGF 165 (Vascular endothelial growth factor, or VEGF-A) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculogenesis and inhibits apoptosis. VEGF 165 mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF 165 is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a prompter of angiogenesis for human gliomas. Human VEGF occurs in several molecular variants and the 165 form is the most common form in most tissues. Biological activities of VEGF 165 are not species specific and glycosylation is not required for biological activity.
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
2xPBS pH 7.2, sterile filtered through 0.22 µm filter
Purity
Greater than 95% by SDS-PAGE gel analysis
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human VEGF 165 is assayed by measuring its dose-dependent effect on the proliferation of HUVEC cells. The ED50 for this effect using VEGF 165 is typically <10 ng/ml. Optimal concentration should be determined for specific applications and cell lines
Endotoxin Level
Endotoxin level is < 0.01 EU per μg of ISOkine® product by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Synonyms
VEGF-A, VEGFA, Vascular endothelial growth factor (VEGF), Vascular Endothelial Growth Factor 165, VEGF165, VEGF 165, Vascular permeability factor (VPF), UniProtKB# P15692.
Documents
Product References
- Cuomo, Francesca et al. “HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring.” Frontiers in cell and developmental biology vol. 9 740203. 12 Jan. 2022, doi:10.3389/fcell.2021.740203
- D'Alessandro, Rosalba et al. “Ramucirumab and GSK1838705A Enhance the Inhibitory Effects of Low Concentration Sorafenib and Regorafenib Combination on HCC Cell Growth and Motility.” Cancers vol. 11,6 787. 7 Jun. 2019, doi:10.3390/cancers11060787
- Cuomo, Francesca et al. “Pro-inflammatory cytokines activate hypoxia-inducible factor 3α via epigenetic changes in mesenchymal stromal/stem cells.” Scientific reports vol. 8,1 5842. 11 Apr. 2018, doi:10.1038/s41598-018-24221-5
- Sharma, Dileep et al. “The effect of bisphosphonates on the endothelial differentiation of mesenchymal stem cells.” Scientific reports vol. 6 20580. 9 Feb. 2016, doi:10.1038/srep20580
- Binder, N K et al. “Endometrial signals improve embryo outcome: functional role of vascular endothelial growth factor isoforms on embryo development and implantation in mice.” Human reproduction (Oxford, England) vol. 29,10 (2014): 2278-86. doi:10.1093/humrep/deu211
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Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.