Generate clinically relevant quantities of iPSCs with beadMATRIX+.
YOUR CHALLENGE
Your aim is to generate clinically relevant quantities of induced pluripotent stem cells (iPSCs) with an expected application in cell-based therapies. You have faced difficulties upscaling your expansion process from 2D to 3D while maintaining cell quality, safety, and potency. Ideally, you would like to boost the efficiency of your manufacturing process while standardizing protocols and complying with safety regulations.
THE SOLUTION
beadMATRIX+ are animal component-free, pre-coated microcarriers specifically designed for bioreactor-scale expansion of iPSCs in serum-free and xeno-free media. The coating formulation is based on the successful 2D coating product myMATRIX iPSC and tailored to support customers in the establishment of translational cell therapies. Documentation describing quality and origin characteristics are provided.
BENEFITS
- Acceleration – enhance the development of your cell-based product.
- Potency – maintain stemness and genetic stability.
- Animal component-free – perform serum-free and xeno-free 3D-culture.
- Compliant – developed in accordance with pharma grade requirements.
Supporting efficient 3D-culture of iPSC
The composition of the beadMATRIX coating has has been further improved to optimally support the expansion of iPSC. iPSCs cultured with beadMATRIX+ proliferate fast and reliable over 3 passages (21 days) while remaining pluripotent and genetically stable. High expression of the stemness genes OCT4, SOX2, SSEA-4, and TRA-1-60 is ensured over multiple passages in a 3D culture setup. The results show that beadMATRIX+ supports dynamic culture of iPSCs and can be implemented for efficient iPSC manufacturing for clinical applications.
Compatible with various manufacturing processes
The upscaling of cell manufacturing is a complex topic. In collaboration with denovoMATRIX, we aim to support the establishment and improvement of existing processes with the help of a highly compatible product. The beadMATRIX products have been successfully tested with different bioreactor setups and several cell culture media. beadMATRIX production procedures ensure batch-to-batch consistency, product stability and compliance with the high-quality standards of ISO9001 and ISO14644. beadMATRIX and beadMATRIX+ documentation addresses pharma grade requirements in respect of material origin and quality. The microcarriers are sterile and ready-to-use and available in 10 gram units with larger sizes available upon request.
SPECIFICATIONS
| Bead material | Cross-linked polystyrene |
| Coating | Biofunctional polymer |
| Surface charge | Slight cationic charge |
| Relative density range | 1.022 – 1.03 g/cm^3 |
| Bead size | 179 μm ± 10% |
| Specific surface area | 330 cm^2/g |
| No. of beads per gram | 3.3 x 10^5 |
| Appearance | Dry, white to off-white powder |
| Safety | Free of Endotoxins/Animal components/BSE/TSE |
| Application | Sterile product, ready-to-use |
| Certification | ISO9001, pharma grade |
| Quality standards | Manufactured in clean room ISO14644-1 class 5 |
| Available formats | 30 g (3 x 10 g). For larger quantities, please request a quote. |
Resources
- beadMATRIX+ Product Info Sheet (PDS)
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Application Note: Efficient microcarrier-assisted 3D culture with
beadMATRIX+
Related Products
- myMATRIX iPSC: precoated cultureware for expansion of iPSCs
- beadMATRIX: precoated microcarriers for MSCs culture
- Serum Replacements: easily replace FBS in your cell cultures!
- Serum-free cell culture media
Ilex Life Sciences LLC is an authorized distributor of denovoMATRIX beadMATRIX+.