myMATRIX iPSC - precoated induced pluripotent stem cells cultureware

C0505X28

Regular price: $1,670.00
Sale price: $1,670.00
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YOUR CHALLENGE

Using animal derived coatings limits the impact of your research on human induced pluripotent stem cells (hiPSC) and impedes the reproducibility of your results. It’s tedious to always freshly coat your plates. You’ve tried a few alternatives which are animal-free, however cell performance suffers, and you end up returning to your previous ‘gold standard’. Ideally, you want the reliability of more defined coatings, while maintaining the performance of your cell culture, without the hassle of pre-coating.

THE SOLUTION

Xeno-free and chemically defined pluripotent stem cell culture with myMATRIX iPSC.

myMATRIX iPSC precoated cultureware plates

myMATRIX iPSC – denovoMATRIX provides a chemically defined cell adhesion-promoting microenvironment offered as pre-coated cultureware, specially formulated for expansion of induced pluripotent stem cells (hiPSC).

BENEFITS

Ready-to-use
Batch-to-batch consistency
Animal component-free product
Typical iPSC morphology and robust proliferation
High and consistent expression of stemness markers
Preservation of pluripotency and genetic stability

RECAPITULATE KEY FUNCTIONS OF THE CELLULAR MICROENVIRONMENT

myMATRIX coatings are manufactured without any animal or human components and are ready-to-use. Human induced pluripotent stem cells have a stable karyotype, high expression of stemness genes and are pluripotent after long-term culture on myMATRIX iPSC. The coating combines biologically relevant synthetic peptides with sulfated glycosaminoglycan (GAG) analogs to recreate the functional aspects of the cellular microenvironment.

Maintaining pluripotency and genetic stability

Consistent and high expression of stemness markers

Consistent and high expression of stemness markers

Surface-antigen expression patterns of iPSCs cultured on myMATRIX iPSC show high expression of pluripotency markers as analyzed by Fluorescence-activated cell sorting (FACS).
iPSCs on myMATRIX show high expression of a series of iPSC-specific genes such as OCT4, Nanog, Sox2, FGF2 and Lin28 throughout the culture estimated by quantitative RT-PCR (see white paper).

Preservation of genetic stability

iPSCs cultured on myMATRIX iPSC are genetically stable.
Normal, healthy karyotypes after long-term culture of 20 passages.

Preservation of differentiation capacity

iPSCs on myMATRIX maintain their differentiation potential and therefore, form all three germ layers after long-term culture.
Cells were differentiated after 20 passages in culture.
Differentiated cells (DAPI, blue) and germ layer-specific marker expression were visualized by immunostaining: Mesoderm – Brachyury (green), CXCR4 (red) Endoderm – GATA6 (green), Sox17 (red) Endoderm – Nestin (green), Pax6 (red).