Description
Recombinant Carboxypeptidase B Rat is a 34.6 kDa protease expressed in Pichia pastoris yeast with specificity for basic amino acids such as lysine and arginine.
Carboxypeptidase B is GMP manufactured in a certified facility with animal component free certified raw materials.
Applications
Carboxypeptidase B hydrolyzes the basic amino acids lysine (L), arginine (R), and ornithine from the C-terminal position of polypeptides.
Carboxypeptidase B is a critical reagent in the manufacturing of human therapeutics such as insulin and monoclonal antibodies (mAbs). It is also a critical reagent in the field of protein analysis, specifically in the precise determination of protein sequences and post-translational modifications.
- Peptide mapping and protein sequencing
- Determination of protein C-terminal amino acid
- Sequence analysis by successive cleavage of C-terminal basic amino acids.
- Therapeutic insulin production by converting pro-insulin to insulin.
- Therapeutic monoclonal antibodies manufacturing and quality control.
Advantages
- Manufactured from TSE/BSE and animal component-free raw materials.
- Manufacturing process is free of antibiotics, melamine, and allergens.
- Pharmacopoeia certified formulation ingredient.
- Manufactured under cGMP standards in ISO 9001:2015 certified facilities with ISO 7 and ISO 8 clean rooms following ICH Q7 principles.
- Halal and Kosher certified manufacturing process.
- Extensive product characterization and documentation.
Characteristics & Specifications
Carboxypeptidase B Rat is provided as a solution with the following specification: 33 mM Tris/HCl, 0.1 mM ZnCl2, pH 7.5-8.5. Each batch is tested and released as per specifications to achieve consistent product quality.
| CAS Registry No. | 9025-24-5 |
| Molecular Weight | 35.2 kDa |
| Appearance | clear, colorless to light yellow solution |
| Formulation | 33 mM Tris/HCl, 0.1 mM ZnCl2 |
| pH Value | 7.5 - 8.5 |
| Activity | ≥ 400 U/ml (with Hippuryl-arginine) |
| Specific Activity | > 230 U/ml (with Hippurly-arginine) |
| Protein Content | 2.8 ± 0.5 mg/ml |
| Purity (RP-HPLC) | ≥ 85% |
| Microbial Count TAMC | < 5 CFU/ml |
| Microbial Count TYMC | < 5 CFU/ml |
| Trypsin Activity | ≤ 0.005% (Chromozyme) |
| Manufacturer | Provis Biolabs |
| Country of Origin | India |
Storage
Carboxypeptidase B Rat is stable at a long term storage temperature of -20°C. Avoid repeated freeze/thaw cycles.
Usage
This product is intended for laboratory research and biomanufacturing use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Other Names
Carboxypeptidase B, Carboxypeptidase-B, Cpb1, Cpb, EC:3.4.17.2, Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase
Compare to Roche Carboxypeptidase B, recombinant.
Resources
Ilex Life Sciences LLC is an authorized distributor of Provis Biolabs pvt Ltd.