Description
Our bioactive Erythropoietin Alpha (EPO) Human, CHO Recombinant Protein is a single, polypeptide chain that contains 166 amino acids and has a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 36-40 kDa. EPO-a is purified by proprietary chromatographic techniques.
Summary
Erythropoietin is a glycoprotein cytokine secreted mainly by the kidneys in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. Erythropoietin is an essential hormone for red blood cell production. Without it, definitive erythropoiesis does not take place. EPO can induce erythroid differentiation in hematopoietic progenitor cells, making it a valuable tool for studying the process of red blood cell development.
Other Names
Erythropoietin-alpha, EPOa, EPO-alpha, EPO-a
Source
Expressed in Chinese hamster ovary (CHO) cells.
Formulation
Sterile-filtered, white lyophilized (freeze-dried) powder. Each mg of lyophilized powder contains 0.59 mg sodium citrate, 0.58 mg sodium chloride, and 0.006 mg citric acid.
Purity
Greater than 98.0% as determined by:
- Analysis by RP-HPLC.
- Analysis by SDS-PAGE.
Biological Activity
The Specific Activity was measured by normocythemic mice bioassay and was found to be 150,000 IU/mg (NOTE: may vary with each lot number. Please refer to the datasheet enclosed with your product).
Amino Acid Sequence
APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR.
Reconstitution
It is recommended to reconstitute the lyophilized EPO-alpha in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized EPO, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, EPO should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.