Introduction
ErythroPlus is a serum-free, premium quality media kit for the culture of erythroid cells. The kit includes a lipid supplement and a supplement with iron-loaded deferiprone that can partially replace holotransferrin. The medium is free of any animal-derived components and contains no phenol red. The kit uses an Iscove's Modified Dulbecco's Medium (IMDM) as the basal medium.
Application
ErythroPlus is a medium especially developed for erythrocyte expansion and differentiation, containing components for optimal growth and proliferation under serum-free conditions. In addition to the two included supplements, it is recommended to add Penicillin/Streptomycin and 2 mM L-Glutamine. For culturing erythroid cells, it is necessary to supplement with application-specific growth factors, such as Erythropoietin and Human Recombinant Stem Cell Factor. Addition of Albumin is not necessary.
Kit Components
|
Component |
Description |
Size |
Storage |
| Basal Medium (P04-20251) |
IMDM, w/o: L-Glutamine, w: Insulin hum. rec., w: PVA, w: 3.024 g/L NaHCO3 | 500 ml | +2°C - +8°C |
| P04-20251L | Lipid Supplement, w: Synthechol, defined, 1000x | 0.5 ml | +2°C - +8°C |
| P04-20251BS | Deferiprone Supplement, 500x | 1 ml | +2°C - +8°C |
Specifications
Cat. No. P04-20251K
| Cell Type: | Erythroid cells (erythrocytes) |
|---|---|
| Bicarbonate: | With 3.024 g/L Sodium Bicarbonate |
| Glutamine: | Without Glutamine |
| Liquid / Powder: | Liquid |
| Phenol Red: | No Phenol Red |
| Product Category: | Serum-Free Media |
| Product Type: | IMDM |
| Size: | Various |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Documents
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