Description
ISOkine® SCF Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 164 amino acids and an N-terminal 6 amino acid histidine tag for a total length of 170 amino acids and has a predicted molecular mass of 19.3 kDa.
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Summary
Stem Cell Factor (SCF) is a hematopoietic growth factor acting at the early stages of hematopoiesis. SCF promotes the survival, proliferation, and differentiation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also stimulates the growth and activation of mast cells and eosinophils. SCF has been shown to act synergistically with other growth factors, such as GM-CSF, G-CSF, IL-7 and EPO. Human SCF shows low activity on murine cells, while murine and rat SCF are fully active on human cells.
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
2xPBS pH 7.2, sterile filtered through 0.22 µm filter
Purity
> 95% by SDS-PAGE gel analysis.
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human SCF is assayed by measuring its dose-dependent effect on the proliferation of TF-1 cells. The ED50 for this effect using SCF is typically <2-10 ng/ml. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Endotoxin level is < 0.01 EU per μg of ISOkine® product by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Synonyms
KIT ligand, c-Kit ligand, KITLG, KL, KL-1, KITL, mast cell growth factor, MGF, Steel factor, SF.
Documents
Product References
- Zhang, Liangying et al. “SOCS2 inhibits the tumorigenesis of GISTs and increases the sensitivity of GISTs to imatinib by suppression of KIT activation.” Scientific reports vol. 15,1 4779. 8 Feb. 2025, doi:10.1038/s41598-025-89477-0
- Zhang, Shaoting et al. “Four and a half LIM domains 2 (FHL2) attenuates tumorigenesis of gastrointestinal stromal tumors (GISTs) by negatively regulating KIT signaling.” Molecular carcinogenesis vol. 63,7 (2024): 1334-1348. doi:10.1002/mc.23727
- Li, Shujing et al. “SPRY4 inhibits and sensitizes the primary KIT mutants in gastrointestinal stromal tumors (GISTs) to imatinib.” Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association vol. 26,5 (2023): 677-690. doi:10.1007/s10120-023-01402-4
- Zhang, Shaoting et al. “Protein tyrosine phosphatase receptor type E (PTPRE) regulates the activation of wild-type KIT and KIT mutants differently.” Biochemistry and biophysics reports vol. 26 100974. 2 Mar. 2021, doi:10.1016/j.bbrep.2021.100974
- Root, Sierra H, and Héctor L Aguila. “Novel population of human monocyte and osteoclast progenitors from pluripotent stem cells and peripheral blood.” Blood advances vol. 5,21 (2021): 4435-4446. doi:10.1182/bloodadvances.2021004552
- Riccetti, Silvia et al. “Modelling West Nile Virus and Usutu Virus Pathogenicity in Human Neural Stem Cells.” Viruses vol. 12,8 882. 12 Aug. 2020, doi:10.3390/v12080882
- Zhu, Guangrong et al. “Loss of PI3 kinase association improves the sensitivity of secondary mutation of KIT to Imatinib.” Cell & bioscience vol. 10 16. 12 Feb. 2020, doi:10.1186/s13578-020-0377-9
- Phung, Bengt et al. “C-KIT signaling depends on microphthalmia-associated transcription factor for effects on cell proliferation.” PloS one vol. 6,8 (2011): e24064. doi:10.1371/journal.pone.0024064
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Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.