Description
The cardiomyocyte maturation reagent.
ISOkine® Neuregulin 1 (NRG1) Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 68 amino acids and an N-terminal 10 amino acid histidine tag for a total length of 78 amino acids and has a predicted molecular mass of 9.3 kDa.
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Applications
- hiPSC-Cardiomyocyte Maturation: Used in maturation protocols for hiPSC-derived cardiomyocytes.
- Cardiac Safety & Disease Modeling: Essential for cardiac safety pharmacology and disease model development.
- Cardiac Organoids & Engineered Tissues: Supports maturation of cardiac organoids and engineered heart tissues.
- Schwann Cell & Peripheral Nerve: Supports Schwann-cell biology and peripheral-nerve regeneration research.
- Cardiac-Oncology Research: Used to study ErbB2 inhibitor toxicity and cardiac homeostasis.
Summary
Neuregulin-1 (NRG-1) promotes cell growth, survival, proliferation and differentiation. NRG-1 is a signaling protein of the EGF family that acts primarily as a direct ligand for the ErbB3 (HER3) and ErbB4 (HER4) receptor tyrosine kinases, which in turn partners with ErbB2 (HER2) to activate downstream PI3K/AKT and MAPK signaling.
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
2xPBS pH 7.2, sterile filtered through 0.22 µm filter
Purity
≥ 95% by SDS-PAGE gel analysis.
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human NRG-1 is assayed by measuring its dose-dependent effect on the proliferation of MCF-7 cells. The ED50 for this effect using NRG-1 is typically ≤2 ng/ml. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Endotoxin level is < 0.05 EU per μg of ISOkine® product by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Synonyms
Neuregulin-1, NRG-1, NRG1, GGF, HGL, HRGA, NDF, SMDF, HRG, ARIA, GGF2, HRG1.
Documents
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Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.