Our bioactive Stem Cell Factor (SCF) Human, HEK Recombinant Protein is a glycosylated monomer with a molecular weight range of 35-45 kDa due to glycosylation. The SCF is purified by proprietary chromatographic techniques.
Stem cell factor (SCF), also known as KIT ligand, binds to the receptor-type protein-tyrosine kinase KIT (CD117). This pleiotropic cytokine plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. SCF exists in both a soluble and a transmembrane isoform. SCF is commonly used in cell cultures in vitro to promote expansion of primitive hematopoietic stem cells and multi-potent progenitor cells.
KIT ligand, c-Kit ligand, KITLG, KL, KL-1, KITL, mast cell growth factor, MGF, Steel factor, SF
Sterile filtered white lyophilized (freeze-dried) powder. The SCF was lyophilized from a 0.2 µm filtered solution (1 mg/ml) containing 1xPBS.
Greater than 95.0% as determined by SDS-PAGE analysis.
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
The EC50 is 15.25 ng/ml.
It is recommended to reconstitute the lyophilized SCF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Lyophilized SCF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
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