Lyophilized vials of Ilex Life Sciences Stem Cell Factor (SCF, Kit Ligand) Human, HEK Recombinant Protein

Stem Cell Factor (SCF) Human, HEK Recombinant Protein

A31110010
Regular price
$195.00
Sale price
$195.00
Regular price
Available January 20th
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per 
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Description

Our bioactive Stem Cell Factor (SCF) Human, HEK Recombinant Protein is a glycosylated monomer with a molecular weight range of 35-45 kDa due to glycosylation. The SCF is purified by proprietary chromatographic techniques.

Summary

Stem cell factor (SCF), also known as KIT ligand, binds to the receptor-type protein-tyrosine kinase KIT (CD117). This pleiotropic cytokine plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. SCF exists in both a soluble and a transmembrane isoform. SCF is commonly used in cell cultures in vitro to promote expansion of primitive hematopoietic stem cells and multi-potent progenitor cells.

Other Names

KIT ligand, c-Kit ligand, KITLG, KL, KL-1, KITL, mast cell growth factor, MGF, Steel factor, SF

Source

HEK cells

Formulation

Sterile filtered white lyophilized (freeze-dried) powder. The SCF was lyophilized from a 0.2 µm filtered solution (1 mg/ml) containing 1xPBS.

Purity

Greater than 95.0% as determined by SDS-PAGE analysis.

Biological Activity

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
The EC50 is 15.25 ng/ml.

    Reconstitution

    It is recommended to reconstitute the lyophilized SCF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

    Shipping

    At ambient temperature.  Upon receipt, store the product at the temperature recommended below.

    Storage/Expiration

    Lyophilized SCF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Usage

    This product is intended for Laboratory Research Use Only. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

    Resources

    Want to learn more about SCF? We have compiled the links below which contain information that you may find interesting.