Description
Serum replacements for cell culture media address the scalability, performance, sustainability and ethical challenges of fetal bovine serum (FBS). However, developing effective alternatives has traditionally been costly and time-consuming. This delays new applications of cell culturing in getting from lab to market.
Multus Biotechnology Ltd. makes development of animal component-free (ACF) media cheaper and faster, delivering affordable, high-performance media for a wider variety of cells. Multus products are designed to help establish scalable processes for animal cell culturing.
The Proliferum® B Panel of four different formulations is designed to support the growth of various bovine and murine cell types, performing best on fibroblasts. Upon determining which of the four formulations works best for your cells, you may purchase that formulation on its own.
Specifications
|
pH: |
7.0 - 9.0 |
| Osmolarity (mOsm/kg): | 290 - 310 |
| Bacteria Testing: | Negative |
| Mycoplasma Testing: | Negative |
| Fungal Testing: | Negative |
| Particulate Examination: | Negative |
| Cell Growth: | Pass |
| Storage: | -20°C, protect from light |
| Manufacturer: | Multus Biotechnology Ltd. |
| Country of Origin: | United Kingdom |
Performance
Figure 1: Average doubling times from ≥ 3 passages (n=2) between 10% FBS and 10% (1X) Proliferum® B in DMEM/F12. For each cell model, the best performing formulation from the panel is presented.
Complete Media Preparation
For cell culturing dilute in basal media (DMEM/F12 recommended) to your desired concentration. We recommend 1-2X but suggest titration experiments to determine the optimal concentration for your cells. Ensure the complete media has reached 25°C prior to cell culturing and minimize light exposure.
|
1 L Proliferum B (1X) |
1 L Proliferum B (2X) |
|
|
Usage: |
Replaces 1 L of 10% FBS | Replaces 1 L of 20% FBS |
|
Proliferum B (10X): |
100 ml | 200 ml |
|
DMEM/F12: |
900 ml | 800 ml |
Adaptation
Switching directly from FBS to Proliferum® B is recommended (with an appropriate tissue culture coating). If cells exhibit poor viability and/or growth, we recommend the following adaptation protocol:
| Passage 1: | 50% FBS medium : 50% Proliferum® B medium |
| Passage 2: | 25% FBS medium : 75% Proliferum® B medium |
| Passage 3: | 0% FBS medium : 100% Proliferum® B medium |
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Other Names
FBS replacement, FBS substitute, serum substitute
Resources
Related Products
Ilex Life Sciences LLC is an authorized distributor of Multus Biotechnology Limited.