PAN-Biotech HybridBoost: Defined Serum-Free Hybridoma Medium, w: stable Glutamine, w: Insulin hum. rec., w/o: Phenol red, w: 2.438 g/L NaHCO3, GMP Grade, 500 ml bottle, cat. no. CT-995905, distributed by Ilex Life Sciences LLC.

PAN-Biotech HybridBoost: Defined Serum-Free Hybridoma Medium, w: stable Glutamine, w: Insulin hum. rec., w/o: Phenol red, w: 2.438 g/L NaHCO3, GMP Grade

CT-995905
$155.00
$155.00
$184.00
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Description

Pan-Biotech HybridBoost is a serum-free and defined culture medium specifically designed for enhanced in vitro growth and proliferation of hybridoma cell lines. The optimized formulation allows the generation of a high yield of monoclonal antibodies (mAb). Because it does not contain hydrolysates and only negligible amounts of animal-derived components (< 0.1% w/v) and proteins (< 0.1% w/v), it is ideal for large-scale production of mAb.

GMP Grade

HybridBoost GMP Grade is a ready-to-use, serum-free and defined cell culture medium designed for direct use in GMP-regulated clinical and industrial applications.

The GMP grade version is based on the proven HybridBoost formulation and is manufactured under strictly controlled GMP conditions. Its defined composition, high batch-to-batch consistency and comprehensive documentation make HybridBoost GMP a true ready-to-use solution, suitable for immediate implementation in development, scale-up and manufacturing workflows.

These features make HybridBoost GMP particularly suitable for scalable, reproducible and GMP-compliant monoclonal antibody production.

Specifications

Cat. No.: CT-995905

    Quality: GMP Grade (EU Annex 1)
    Sodium Bicarbonate: With 2.438 g/L sodium bicarbonate
    Cell Type: Hybridoma
    Culture Type: Suspension Culture
    Glutamine: With Stable Glutamine
    Liquid / Powder: Liquid
    Phenol Red: Without phenol red
    Product Category: Serum-Free Media
    Size:

    500 ml
    Sterile: Yes
    Storage Temperature: +2°C - +8°C, in the dark
    Manufacturer: PAN-Biotech GmbH
    Country of Origin: Germany

    Composition

    HybridBoost is formulated with stable Glutamine and is free of phenol red. Additionally, it includes a surfactant, eliminating the need of additional supplements for agitated suspension culture.

    Suitability

    HybridBoost is a ready-to-use, defined medium for serum-free cultivation of hybridoma cell lines at 37 °C in a humidified atmosphere with 5% CO2.

    HybridBoost is highly efficient for diverse hybridoma systems. Cholesterol-dependent cell lines should be supplemented with a lipoprotein preparation or other cholesterol sources. HybridBoost is compatible with several antibiotics, including Penicillin/Streptomycin, Gentamicin, Puromycin, or Fungizone. Nevertheless, the use of antibiotics may lead to a decrease in the yield and efficiency of hybridoma cell lines.

    Figure 1: Comparison of the growth of Sp2/0-Ag14 cells in different serum-free media. The experiment involved seeding 1000 cells/mL and monitoring the results after direct adaption and four days of cultivation.
    Figure 1: Comparison of the growth of Sp2/0-Ag14 cells in different serum-free media. The experiment involved seeding 1000 cells/mL and monitoring the results after direct adaption and four days of cultivation. 

      Storage conditions & stability

      Storage: at +2°C - +8°C in the dark.

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        Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.