Description
PAN-Biotech ISF-1 Serum-Free Hybridoma Medium, w: stable Glutamine, w: Insulin human, w: 2.438 g/L NaHCO3 is a serum-free and defined culture medium for in vitro cultivation and expansion of hybridoma cell lines. High yield and final concentration of monoclonal antibodies (mAb) can be achieved. As this product is free of hydrolysates, and only contains traces of animal-derived components (<0.1% w/v) and proteins (<0.1% w/v), it is suitable for the production of mAb in higher scales.
GMP Grade
ISF-1 GMP Grade is a ready-to-use, serum-free and defined cell culture medium designed for direct use in GMP-regulated clinical and industrial applications.
The GMP grade version is based on the proven ISF-1 formulation and is manufactured under strictly controlled GMP conditions. Its defined composition, high batch-to-batch consistency and comprehensive documentation make ISF-1 GMP a true ready-to-use solution, suitable for immediate implementation in development, scale-up and manufacturing workflows.
These characteristics make ISF-1 GMP particularly suitable for scalable and reproducible monoclonal antibody production in biopharmaceutical manufacturing.
Applications
- Serum-free cultivation of hybridoma cell lines.
- Monoclonal antibody (mAb) production.
- Biopharmaceutical development and manufacturing.
- Scale-up in spinner flasks, roller bottles and bioreactors.
- GMP-compliant antibody production platforms.
Features
- Manufactured in compliance with Good Manufacturing Practice (GMP).
- Serum-free and defined formulation.
- Ready-to-use – immediately applicable.
- High batch-to-batch consistency and process reliability.
Specifications
Cat. No.: CT-995968
| Quality: | GMP Grade (EU Annex 1) |
|---|---|
| Cell Type: | Hybridoma |
| Culture Type: | Suspension Culture |
| Liquid / Powder: | Liquid |
| Sodium Bicarbonate: | With 2.438 g/L Sodium Bicarbonate |
| Phenol Red: | With Phenol Red |
| Product Category: | Serum-Free Media |
| Size: |
1000 ml |
| Sterile: | Yes |
| Storage: | +2°C - +8°C, in the dark. |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Composition
PAN-Biotech ISF-1 Serum-free Hybridoma Medium, w: stable Glutamine, w: Insulin human, w: 2.438 g/L NaHCO3: ISF-1 has been manufactured with stable Glutamine. It also contains a surfactant. Therefore no additional supplement for agitated suspension culture is needed.
Suitability
Serum-free, defined hybridoma culture for production of monoclonal antibodies (mAb).
ISF-1 works well for a variety of hybridoma systems, but will not grow cholesterol dependent cell lines without further supplementation (Addition of a lipoprotein preparation or other source of cholesterol is needed for these cell lines). ISF-1 is compatible with most antibiotics, including Penicillin/Streptomycin, Gentamicin, Puromycin or Fungizone. The use of antibiotics could reduce the yield and productivity of hybridoma cell lines.
This product has been tested in different cell culture system, including stationary culture, spinner, roller bottles and bioreactors.
Figure 1.: Influence of different media on cell growth, viability and productivity. Hybridoma cells were cultivated in T-flasks. After adaption of the cells to the different media, cell growth, viability and productivity were measured after one week of cultivation. Productivity is shown in comparison to 10 % FBS-containing medium.
Storage conditions & stability
Storage: at +2°C - +8°C in the dark.
Related Products
- RUO Version: PAN-Biotech ISF-1 Serum-Free Hybridoma Medium, w: stable Glutamine, w: Insulin human, w: 2.438 g/L NaHCO3
- PAN-Biotech GMP Grade Cell Culture Products
- PAN-Biotech HybridBoost: Defined Serum-Free Hybridoma Medium
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Serum-free cell culture media
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