Description
PAN-Biotech HybridBoost: Serum-free, defined medium for hybridoma cells, w: stable Glutamine, w: Insulin hum. rec., w/o: Phenol red, w: 2.438 g/L NaHCO3.
HybridBoost is a serum-free and defined culture medium specifically designed for enhanced in vitro growth and proliferation of hybridoma cell lines. The optimized formulation allows the generation of a high yield of monoclonal antibodies (mAb). Because it does not contain hydrolysates and only negligible amounts of animal-derived components (< 0.1% w/v) and proteins (< 0.1% w/v), it is ideal for large-scale production of mAb.
| Sodium Bicarbonate: | With 2.438 g/L sodium bicarbonate |
|---|---|
| Cell Type: | Hybridoma |
| Culture Type: | Suspension Culture |
| Glutamine: | With Stable Glutamine |
| Liquid / Powder: | Liquid |
| Phenol Red: | Without phenol red |
| Product Category: | Serum-Free Media |
| Size: |
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| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Composition
HybridBoost is formulated with stable Glutamine and is free of phenol red. Additionally, it includes a surfactant, eliminating the need of additional supplements for agitated suspension culture.
Suitability
HybridBoost is a ready-to-use, defined medium for serum-free cultivation of hybridoma cell lines at 37 °C in a humidified atmosphere with 5% CO2.
HybridBoost is highly efficient for diverse hybridoma systems. Cholesterol-dependent cell lines should be supplemented with a lipoprotein preparation or other cholesterol sources. HybridBoost is compatible with several antibiotics, including Penicillin/Streptomycin, Gentamicin, Puromycin, or Fungizone. Nevertheless, the use of antibiotics may lead to a decrease in the yield and efficiency of hybridoma cell lines.
Storage conditions & stability
Storage: at +2°C - +8°C in the dark.
Stability: 1 year from date of production
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Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.