PAN-Biotech Trypsin 0.05% / EDTA 0.02% in PBS, w/o: Ca and Mg, 100 ml bottle, cat. no. P10-023100, distributed by Ilex Life Sciences LLC
PAN-Biotech Trypsin 0.05% / EDTA 0.02% in PBS, w/o: Ca and Mg, 500 ml bottle, cat. no. P10-023500, distributed by Ilex Life Sciences LLC
  • Load image into Gallery viewer, PAN-Biotech Trypsin 0.05% / EDTA 0.02% in PBS, w/o: Ca and Mg, 100 ml bottle, cat. no. P10-023100, distributed by Ilex Life Sciences LLC
  • Load image into Gallery viewer, PAN-Biotech Trypsin 0.05% / EDTA 0.02% in PBS, w/o: Ca and Mg, 500 ml bottle, cat. no. P10-023500, distributed by Ilex Life Sciences LLC

PAN-Biotech Trypsin 0.05% / EDTA 0.02% in PBS, w/o: Ca and Mg

P10-023100
$35.00
$35.00
$41.00
Unavailable
Shipping calculated at checkout.

Description

PAN-Biotech Trypsin 0.05% / EDTA 0.02% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture.

Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts.

    Suitability

    Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application.

    Specifications

    Cat. No. P10-023100, P10-023500

    Ca/Mg Content: Without Ca and Mg
    Concentration
    		 1x
    Trypsin Concentration 0.05% Trypsin
    EDTA Concentration
    		 0.02% EDTA
    Liquid/Powder
    		 Liquid
    Phenol Red Without phenol red
    Product Category Enzymes for cell dissociation
    Product Type Trypsin-EDTA
    Size Various
    Sterile Yes
    Storage Temperature -20°C
    In Solution In PBS
    Contaminants Tested negative for mycoplasma and PPV.
    Manufacturer PAN-Biotech GmbH
    Country of Origin Germany

    Application Note

    Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.

    Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.

    Resources

    Product References

    • Dörschmann, Philipp et al. “Anti-inflammatory properties of antiangiogenic fucoidan in retinal pigment epithelium cells.” Heliyon vol. 9,4 e15202. 10 Apr. 2023, doi:10.1016/j.heliyon.2023.e15202
    • Priester, Jasmin et al. “Metabolic labeling and LC-MS/MS-based identification of interleukin-1α-induced secreted proteomes from epithelial cells in the presence or absence of serum.” STAR protocols, vol. 4,2 102195. 31 Mar. 2023, doi:10.1016/j.xpro.2023.102195
    • Flegel, Jana et al. “The Highly Potent AhR Agonist Picoberin Modulates Hh-Dependent Osteoblast Differentiation.” Journal of medicinal chemistry vol. 65,24 (2022): 16268-16289. doi:10.1021/acs.jmedchem.2c00956
    • De Maio, Flavio et al. “Graphene nanoplatelet and graphene oxide functionalization of face mask materials inhibits infectivity of trapped SARS-CoV-2.” iScience vol. 24,7 (2021): 102788. doi:10.1016/j.isci.2021.102788
    • Hörner, Maximilian et al. “Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-associated viral vectors.” Science advances vol. 7,25 eabf0797. 16 Jun. 2021, doi:10.1126/sciadv.abf0797
    • Boussicault, Lydie et al. “Combination of acamprosate and baclofen (PXT864) as a potential new therapy for amyotrophic lateral sclerosis.” Journal of neuroscience research vol. 98,12 (2020): 2435-2450. doi:10.1002/jnr.24714
    • Koepke, Lennart et al. “An improved method for high-throughput quantification of autophagy in mammalian cells.” Scientific reports vol. 10,1 12241. 22 Jul. 2020, doi:10.1038/s41598-020-68607-w
    • Kienzl, Melanie et al. “IL-33 reduces tumor growth in models of colorectal cancer with the help of eosinophils.” Oncoimmunology vol. 9,1 1776059. 16 Jun. 2020, doi:10.1080/2162402X.2020.1776059
    • Goldstein, Jérémie D et al. “IL-36 signaling in keratinocytes controls early IL-23 production in psoriasis-like dermatitis.” Life science alliance vol. 3,6 e202000688. 28 Apr. 2020, doi:10.26508/lsa.202000688
    • Pichla, Monika et al. “Nitroxide Radical-Containing Redox Nanoparticles Protect Neuroblastoma SH-SY5Y Cells against 6-Hydroxydopamine Toxicity.” Oxidative medicine and cellular longevity vol. 2020 9260748. 24 Apr. 2020, doi:10.1155/2020/9260748
    • Hu, Bodan et al. “Photoactivable Cholesterol as a Tool to Study Interaction of Influenza Virus Hemagglutinin with Cholesterol.” Bio-protocol vol. 10,4 e3523. 20 Feb. 2020, doi:10.21769/BioProtoc.3523
    • Gan, Qini et al. “Effects of Amylin Against Amyloid-β-Induced Tauopathy and Synapse Loss in Primary Neurons.” Journal of Alzheimer's disease : JAD vol. 70,4 (2019): 1025-1040. doi:10.3233/JAD-190161
    • Baaske, Julia et al. “Optogenetic control of integrin-matrix interaction.” Communications biology vol. 2 15. 8 Jan. 2019, doi:10.1038/s42003-018-0264-7
    • Lemus-Diaz, Nicolas et al. “Dual Fluorescence Reporter Based Analytical Flow Cytometry for miRNA Induced Regulation in Mammalian Cells.” Bio-protocol vol. 8,17 e3000. 5 Sep. 2018, doi:10.21769/BioProtoc.3000
    • Böker, Kai O et al. “The Impact of the CD9 Tetraspanin on Lentivirus Infectivity and Exosome Secretion.” Molecular therapy : the journal of the American Society of Gene Therapy vol. 26,2 (2018): 634-647. doi:10.1016/j.ymthe.2017.11.008
    • Servin-Vences, Martha Rocio et al. “Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes.” eLife vol. 6 e21074. 30 Jan. 2017, doi:10.7554/eLife.21074

    Related Products

      Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.