PAN-Biotech Trypsin 0.05% / EDTA 0.02% in DPBS, w/o: Ca and Mg, GMP Grade

Description

PAN-Biotech Trypsin 0.05% / EDTA 0.02% in DPBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a DPBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture.

Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts.

GMP Grade

Trypsin 0.05% / EDTA 0.02% GMP Grade is a ready-to-use enzymatic cell detachment solution designed for direct use in GMP-regulated clinical and industrial applications. It enables gentle and reproducible dissociation of adherent cells and tissues, supporting controlled and robust cell processing workflows.

The moderate trypsin concentration makes this formulation particularly suitable for sensitive cell lines and controlled detachment procedures, as required in clinical cell processing, cell and gene therapy, and biopharmaceutical manufacturing.

The GMP grade version is based on the well-established trypsin-EDTA formulation and is manufactured under strictly controlled GMP conditions. Combined with high batch-to-batch consistency, defined quality parameters and comprehensive documentation, this product represents a true ready-to-use solution suitable for immediate implementation in sensitive manufacturing and clinical processes.

Applications

• Detachment of adherent cells under GMP conditions.
• Clinical cell processing and cell therapy.
• Cell and gene therapy workflows.
• Biopharmaceutical development and manufacturing.
• Reproducible cell passaging and harvesting.

Features

• Manufactured in compliance with Good Manufacturing Practice (GMP).
• Ready-to-use – immediately applicable.
• Controlled and gentle cell detachment.
• High batch-to-batch consistency and process reliability.

Suitability

Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application.

Specifications

Cat. No. CT-023100

Application Note

Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.

Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells. For inhibiting trypsin activity in serum-free culture of adherent cells, we recommend using PAN-Biotech Trypsin Inhibitor III.

Related Products

• RUO Version: PAN-Biotech Trypsin 0.05% / EDTA 0.02% in DPBS, w/o: Ca and Mg
• PAN-Biotech GMP Grade Cell Culture Products
• Enzymes for cell dissociation
  
• Bovine Trypsin, recombinant, expressed in corn, lyophilized powder

Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.