Introduction
Williams and Gunn originally developed William's E Medium as a reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. William's E Medium may also be used for the cultivation of primary hepatocyte cells of other species as well as human-derived HepaRG cells. The medium is unique in that it contains zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione, and the lipid methyl linoleate.
Composition
PAN-Biotech William's Medium E, w/o: L-Glutamine, w/o: Phenol red, w: 2.24 g/L NaHCO3
Specifications
Cat. No. P04-29510
| Bicarbonate: | With 2.24 g/L Sodium Bicarbonate |
|---|---|
| Endotoxin Content: | Endotoxin Content unspecified |
| Glucose: | With glucose |
| Glutamine: | No Glutamine |
| HEPES: | Without HEPES |
| Liquid / Powder: | Liquid |
| Phenol Red: | Without Phenol Red |
| Product Category: | Cell Culture Media |
| Product Type: | William's Medium E |
| Size: | Various |
| Sodium Pyruvate: | With Sodium Pyruvate |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Documents
Product References
- Karaca, Mawien et al. “Adverse outcome pathway-based analysis of liver steatosis in vitro using human liver cell lines.” STAR protocols vol. 4,3 (2023): 102500. doi:10.1016/j.xpro.2023.102500
- Demir, Sevgican et al. “TSC22D4 interacts with Akt1 to regulate glucose metabolism.” Science advances vol. 8,42 (2022): eabo5555. doi:10.1126/sciadv.abo5555
- Godoy, Patricio et al. “Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME.” Archives of toxicology vol. 87,8 (2013): 1315-530. doi:10.1007/s00204-013-1078-5
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