Description
PNGase F Recombinant (cloned from Elizabethkingia miricola) expressed in E. coli.
Peptide N-glycosidase F (PNGase F) is an endoglycosidase that removes N-linked glycan oligosaccharides from glycoproteins. PNGase F is considered to be the most effective enzymatic method for removing almost all types of N-linked oligosaccharides from glycoproteins. It is used in the quality control of biotechnological products such as monoclonal antibodies (mAbs) and glycosylated proteins.
Applications
- Glycoprotein analysis
- Protein structure determination
- Antibody deglycosylation
- Amino acids sequence determination
- X-Ray crystallography
- MALDI-TOF or other mass spectrometry techniques
Advantages
- Animal-derived component free.
- Expressed using recombinant DNA technology, ensuring high purity and quality.
- Pharmacopoeia certified formulation ingredient.
- Manufactured under cGMP standards in ISO 9001:2015 certified facilities.
- Extensive product characterization and documentation.
Characteristics & Specifications
| CAS Registry No. | 83534-39-8 |
| Molecular Weight | 36 kDa |
| Purity (SDS-PAGE) |
≥95% |
| Potency |
10 KU/mL |
| Optimal pH | 7.5 - 8.5 |
| Manufacturer | Provis Biolabs |
| Country of Origin | India |
Storage
- +2°C/+8°C up to 12 months
- -20°C up to 24 months
Usage
This product is intended for laboratory research and biomanufacturing use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Other Names
Elizabethkingia miricola (Chryseobacterium miricola) Peptide-N(4)-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F, PNGase F enzyme, PNGaseF, Glycopeptide N-glycosidase, N-glycanase, N-Glycosidase F, Peptide N-glycosidase, EC:3.5.1.52
Resources
Ilex Life Sciences LLC is an authorized distributor of Provis Biolabs pvt Ltd.