Description
Provis Biolabs Recombinant Human Albumin (rHA), expressed in Pichia pastoris, is a synthetic alternative to human serum albumin (HSA). The non-glycosylated, polypeptide chain contains 585 amino acids and has a molecular mass of 67 kDa. The optimum concentration for recombinant albumin to be used in cell culture ranges between 0.5 g - 2 g per liter. The recombinant albumin is purified by chromatographic techniques. It does not contain any animal-derived components, thus offering a reliable and sustainable alternative to traditional HSA sources.
Provis Biolabs Recombinant Human Albumin (rHA) is manufactured under ISO 9001:2015 quality system. Animal-origin-free (AOF). Suitable for research use only (RUO) applications. GMP Grade is available upon request (made-to-order custom production).
Features
- Available as a lyophilized powder or as a liquid solution.
- Expressed in yeast P. pastoris by recombinant DNA technology.
- Manufactured by yeast fermentation process and purified using chromatography methods.
- Purity levels >98% (SDS-PAGE, HPLC).
- Antibiotic-free and solvent-free manufacturing process.
- Raw materials used are animal component free, TSE/BSE-free certified, and comply with IP/USP/EP.
- Manufactured in a GMP certified facility with ISO classified clean rooms, following ICH Q7 principles to meet international standards.
- Quality control testing as per ICH Q6 and Q1 guidelines.
- Product is packed in single-use sterile endotoxin-free containers.
Applications
Due to free hydrophobic regions albumins are used to solubilize lipids and stabilize other proteins such as e.g. growth factors in tissue culture applications. They are also used as blocking agents in Western blots or ELISA applications and as a protein standard. Albumin has a low molecular weight, is easily soluble in water and contains an excess of acidic amino acids.
- Cell and tissue culture media supplement
- Used as serum substitute in serum-free, xeno-free cell culture
- Medium component for cryopreservation and stem cell culture
- Stabilizer protein for pharmaceutical and biotech products
- Reduces unspecific binding in molecular biology assays
Specifications
| Quality: | Research Use Only (RUO). GMP Grade is available on request. |
| Product Category: |
Media supplements |
| Product Type: |
HSA / BSA |
|
CAS Registry No.: |
70024-90-7 |
| Source: |
Expressed in Pichia pastoris yeast (animal-free, non-glycosylated) |
| Liquid / Powder: |
Lyophilized powder |
| Appearance: |
An off-white to pale yellow powder |
| Purity: |
>98% (SDS-PAGE, HPLC) |
| Endotoxin: |
<0.125 EU/mg |
| Storage Temperature: | +2°C - +8°C |
| Optimal Concentration (Cell Culture): | 0.5 – 2.0 g/L |
| Manufacturer: |
Provis Biolabs pvt Ltd |
| Country of Origin: |
India |
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
Background
Albumins are monomeric proteins found in body fluids and tissues. Human serum albumin (HSA) is the most abundant protein in human blood plasma with a molecular weight of about 66.5 kDa. The major biological function of albumin is to regulate the colloidal osmotic blood pressure. In addition, albumin acts as carrier protein for hormones, fatty acids and other compounds, and buffers pH. The reference range for albumin concentration in serum is approximately 35-50 g/L.
About Provis Biolabs
Provis Biolabs is a fast-growing biotechnology company specializing in premium bioreagents for global pharmaceutical and biopharmaceutical applications. Since founding in 2019, Provis Biolabs has commercialized 10 products with a robust and expanding pipeline. Provis Biolabs has end-to-end capabilities covering R&D to GMP manufacturing, supported by WHO-GMP, GLP, ISO 9001:2015, Halal and Kosher certifications. Leveraging their state-of-the-art fermentation facility, Provis Biolabs provides scalable production solutions to partners worldwide. Combining scientific expertise with operational excellence, Provis Biolabs delivers reliable and high-quality products designed to meet the unmet needs of life sciences research and therapeutic development.
Related Products
Ilex Life Sciences LLC is an authorized distributor of Provis Biolabs.