Description
Transferrin Human Recombinant, expressed in rice, lyophilized powder is a non-glycosylated, polypeptide chain containing 679 amino acids. The transferrin protein has a molecular mass of 76 kDa and is purified by proprietary chromatographic techniques.
Summary
Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71. Transferrin appears to be indispensable for most cells growing in tissue culture.
It is frequently referred to as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis. Apart from its role as an iron transport protein, transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity.
Transferrin is a crucial component for the cultivation of mammalian cells in-vitro. Transferrin is used as a detoxicant in cell culture media by binding contaminating metal ions. Transferrin is often used as a nutrient in fermentation media for recombinant protein and biopharmaceutical production.
Specifications
| Product Category: |
Media supplements |
| Product Type: |
Protein |
|
CAS Registry No.: |
11096-37-0 |
| Source: |
Expressed in Rice Grain plant (Oryza sativa) |
| Appearance: |
Sterile-filtered, lyophilized powder. |
| Formulation: | The protein solution (1 mg/ml) was lyophilized with no additives. |
| Purity (SDS-PAGE): |
97% |
| Iron Content: | The average amount of Ferric Iron is 0.4-0.5 µg per mg of protein (protein concentration is measured by absorption at 280 nm). |
| Biological Activity: | One mg of Recombinant Human Transferrin will bind to approximately 2 µg of Fe. |
| Reconstitution: | Stock solutions can be prepared by dissolving gently into PBS for several minutes. Recommended stock concentrations are 5 mg/ml to 20 mg/ml in PBS, though others can be used as well. Please try to avoid the formation of bubbles when dissolving the protein. Sterile filter through 0.2 µm filter. |
| Shipping Condition: | Shipped at ambient temperature. Upon receipt, store the product at the temperature recommended below. |
| Storage Temperature: | Recombinant transferrin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, it should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Documents
Other Names
Recombinant Human Transferrin, TF, Plant Recombinant Transferrin, Serotransferrin, Siderophilin, Beta-1 metal-binding globulin