Description
Recombinant Human Insulin Protein, produced in E. coli, is a two chain, non-glycosylated polypeptide hormone containing 51 amino acids and has a molecular mass of 5807 Dalton. Insulin is purified by proprietary chromatographic techniques.
Summary
Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the INS gene. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver (UniProtKb_P01308). Recombinant Human Insulin is a commonly used media supplement in mammalian cell culture.
CAS Registry No.
11061-68-0
Purity
Purity greater than 98.0% as determined by RP-HPLC analysis.
Biological Activity
The biological activity was determined to be 28 units/mg.
Formulation
Sterile-filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) solution with no additives.
Reconstitution
Shipping
Ships at ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage
Lyophilized Insulin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Insulin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.