Description
Holo Transferrin Human, Native Protein (Serum) is a glycoprotein purified from human serum that has a molecular mass of approximately 77 kDa. It has a saturated iron content of ~1232 ppm.
Summary
When not bound to iron, transferrin is known as "apotransferrin". When saturated with iron, transferrin is known as “holotransferrin”.
Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71. Transferrin appears to be indispensable for most cells growing in tissue culture.
It is frequently referred to as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis. Apart from its role as an iron transport protein, transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity.
Transferrin is a crucial component for the cultivation of mammalian cells in-vitro. Human Transferrin is used as a detoxicant in media by binding contaminating metal ions. Human Transferrin is often used as a nutrient in fermentation media for recombinant protein and biopharmaceutical production. Additional common uses for Human Transferrin are affinity purification of anti-human transferrin antibodies and also for receptor mediated transfection of molecules (such as DNA) into cells.
Other Names
Human holotransferrin, iron-saturated transferrin, Serotransferrin, Transferrin, Siderophilin, Beta-1-metal-binding globulin, TF, PRO1557, PRO2086, DKFZp781D0156, Holo-Transferrin, HTF.
Source
Human serum
Formulation
Sterile filtered pink lyophilized (freeze-dried) powder. The protein (10 mg/ml) was lyophilized from 20mM NH4HC03 solution. May contain traces of buffer salts.
Purity
Greater than 98.0% as determined by Coomassie blue stained SDS-PAGE and Cellulose Acetate electrophoresis.
Iron Content
The iron content was estimated by ICP and was found to be 1232 ppm.
Reconstitution
It is recommended to reconstitute the lyophilized Holo Transferrin in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Human Virus Test
Plasma from each donor has been tested and found negative for antibodies to HIV-1, HIV-2, HCV, HBsAG, HBc, HBV, HAV, HIV and Syphilis.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the lyophilized Holo Transferrin between 2-8°C, do not freeze.
Upon reconstitution, Holo Transferrin should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.