Biocolor Purple-Jelley™ Hyaluronic Acid Assay - Frequently Asked Questions (FAQ)

 

Here you will find answers to many of the frequently asked questions about Biocolor Purple-Jelley™ Hyaluronic Acid Assay.

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What is the Purple-Jelley Hyaluronic Acid Assay?

The Biocolor Purple-Jelley Hyaluronic Acid Assay is a quantitative, dye-based method for measurement of hyaluronic acid/hyaluronate/hyaluronan derived from tissues.

General Questions

What is the sensitivity of the Purple-Jelley Hyaluronic Acid Assay?

0.2 µg in 20 µl of assayed sample, equivalent to 10 µg/ml.

What absorbance values can samples be measured at?

The absorbance peak for hyaluronic acid in Purple-Jelley Dye Reagent is 655 nm.

Sample Processing

How do I prepare samples for measurement using the Purple-Jelley Hyaluronic Acid Assay?

Preparation details for samples in tissue are described in the assay manual. Removal of tissue protein before assay is essential in order to isolate the HA from potentially dye-binding proteins. Sulfated glycosaminoglycans must also be removed before measuring the isolate HA. This is achieved using a two step critical electrolyte salting out process (CEC).