Description
3-D Life 10x CB-Buffer (pH 7.2) Phenol Red-Free is a component of the 3-D Life Hydrogel system. In order to avoid interferences with light microscopy analysis, this buffer does not contain Phenol Red. It is a 10x concentrated salt and buffer solution which is diluted to physiological strength when used in the 3-D Life Hydrogel system. It is used for the formation of slow gelling 3-D Life Hydrogels where it provides a cell compatible environment at pH 7.2. In conjunction with 3-D Life 10x CB-Buffer (pH 5.5) it is used to generate pHs of intermediate values to modify the gelation kinetics of fast gelling gels.
For instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
Used with other components of the 3-D Life Hydrogel System to establish 3D cell cultures.
Quantity
600 µl.
Sufficient for at least 6 ml 3-D Life Hydrogel.
Resources
- Product Datasheet (PDS): 10x CB Buffer (pH 7.2) Phenol Red-Free
- 3-D Life User Guide & Protocols
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH products.