Description
3-D Life 10x CB-Buffer (pH 5.5) is a component of the 3-D Life Hydrogel system. It is a 10x concentrated salt and buffer solution which is diluted to physiological strength when used in the 3-D Life Hydrogel system. It is used for the formation of fast gelling 3-D Life Hydrogels where it provides a cell environment at pH 5.5, which is compatible with most cell types. In conjunction with 3-D Life 10x CB-Buffer (pH 7.2) it is used to generate pHs of intermediate values to modify the gelation kinetics of fast gelling gels.
For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
Used with other components of the 3-D Life Hydrogel System to establish 3D cell cultures.
Quantity
600 µl.
Sufficient for at least 6 ml 3-D Life Hydrogel.
Resources
- Product Datasheet (PDS): 10x CB-Buffer (pH 5.5)
- Material Safety Data Sheet (MSDS): 10x CB-Buffer (pH 5.5)
- Protocol: Preparation of 3-D Life Fast (FG) Gelling Hydrogels
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Product References
- Wang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” Materials (Basel, Switzerland) vol. 12,20 3391. 17 Oct. 2019, doi:10.3390/ma12203391
- Mohammadi Ayenehdeh, Jamal et al. “Introducing a New Experimental Islet Transplantation Model using Biomimetic Hydrogel and a Simple High Yield Islet Isolation Technique.” Iranian biomedical journal vol. 21,4 (2017): 218-27. doi:10.18869/acadpub.ibj.21.4.218
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH products.