Description
3-D Life CD-HyLink is a component of the 3-D Life Hydrogel system. CD-HyLink consists of hyaluronic acid (hyaluronan) carrying thiol groups along its backbone. When combined with SG-Dextran of the 3-D Life Hydrogel system, thiol groups on CD-HyLink form stable thioether bonds with the thiol-reactive groups on the polymer, which results in the formation of the hydrogel.
CD-HyLink contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13. It allows cells to locally degrade the hydrogel.
Note: Dextran hydrogels crosslinked with CD-HyLink cannot be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1).
For using this product please consult General Protocol GP-4B "Preparation of 3-D Life Hyaluronic Acid (CD-HA) Hydrogels containing a matrix metalloproteinase cleavage site" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
3-D Life CD-HyLink is used together with thiol-reactive SG-Dextran of the 3-D Life Hydrogel system to establish 3D cell cultures.
Quantity
Cat. No. L80-1: Sufficient for 1.6 ml hydrogel of a crosslinking strength of 1.2 mmol/l when used with SG-Dextran of the 3-D Life Hydrogel system.
Cat. No. L80-3: Sufficient for 4.8 ml hydrogel of a crosslinking strength of 1.2 mmol/l when used with SG-Dextran of the 3-D Life Hydrogel system.
Components
Cat. No. L80-1:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| CD-HyLink, Lyophilized |
200 µl* | 10 mmol/L* | MSDS |
| Water | 600 µl | n/a | MSDS |
| *Volume after reconstitution of lyophilizate. |
*Concentration after reconstitution of lyophilizate. |
Cat. No. L80-3:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| CD-HyLink, Lyophilized | 3 x 200 µl* | 10 mmol/L* | MSDS |
| Water | 600 µl | n/a | MSDS |
| *Volume after reconstitution of lyophilizate. | *Concentration after reconstitution of lyophilizate. |
Resources
- Product Datasheet (PDS): 3-D Life CD-HyLink (200 µl)
- Product Datasheet (PDS): 3-D Life CD-HyLink (3 x 200 µl)
-
3-D Life User Guide & Protocols
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Product References
- Kromidas, Elena et al. “Immunocompetent PDMS-Free Organ-on-Chip Model of Cervical Cancer Integrating Patient-Specific Cervical Fibroblasts and Neutrophils.” Advanced healthcare materials vol. 13,21 (2024): e2302714. doi:10.1002/adhm.202302714
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.