3-D Life SG-Dextran Hydrogel Component

3-D Life SG-Dextran Hydrogel Component

M91-3
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$262.00
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Description

3-D Life SG-Dextran is a component of the 3-D Life Hydrogel system. It contains dextran functionalized with thiol-reactive groups (SG-Dextran) that can be used together with PEG-Link (Cat. No. L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up slow gelling, cell-compatible hydrogels.

Dextran-based 3-D Life hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells.

For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.

Application

3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link).

Components

Material Quantity Concentration of reactive groups
SG-Dextran
3 x 170 µl 30 mmol/L MSDS
10x CB Buffer (pH 7.2) 600 µl n.a. MSDS
Water 4 x 1500 µl n.a. MSDS

 Resources

Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.