Description
RGD sequences are motifs on extracellular matrix proteins promoting adhesion to cellular integrin receptors. The 3-D Life Scrambled RGD Peptide contains a scrambled RGD motif and a thiol group on the N-terminus. The thiol group is used to immobilize the peptide on maleimide polymers in 3-D Life Hydrogels. The immobilized peptide is used in control experiments to determine the specificity of cell adhesion caused by a genuine RGD peptide (e.g. 3-D Life RGD Peptide).
For instructions, please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
3D cell culture: Covalent attachment to thiol-reactive polymer in 3-D Life Hydrogels. For control experiments to determine specificity of cell adhesion to RGD Peptide.
Quantity
Cat. No. 09-P-003: 1 µmol
Cat. No. P11-3: 3x 1 µmol
Components
Cat. No. 09-P-003:
Material | Quantity | Concentration of reactive groups | |
---|---|---|---|
Scrambled RGD Peptide, lyophilized | 1 µmol | 20 mmol/L* | MSDS |
Water | 600 µl | n.a. | MSDS |
*Volume/concentration after reconstitution of lyophilizate. |
Cat. No. P11-3:
Material | Quantity | Concentration of reactive groups | |
---|---|---|---|
Scrambled RGD Peptide, lyophilized | 3 x 1 µmol | 20 mmol/L* | MSDS |
Water | 600 µl | n.a. | MSDS |
*Volume/concentration after reconstitution of lyophilisate. |
Peptide sequence
Acetyl-Cys-Doa*-Doa-Gly-Arg-Asp-Gly-Ser-Pro-NH2
*: Doa:8-amino-3,6-dioxaoctanoic acid
Molar mass
1022 g/mol
Purity
>90% (HPLC)
Resources
- Product Datasheet (PDS): 3-D Life Scrambled RGD Peptide (1 µmol)
- Product Datasheet (PDS): 3-D Life Scrambled RGD Peptide (3x 1 µmol)
- Protocol: Preparation of 3-D Life Fast (FG) Gelling Hydrogels
- Protocol: Preparation of 3-D Life Slow Gelling (SG) Hydrogels
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Application Notes
- Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels
- Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.