Description
RGD sequences are motifs on extracellular matrix proteins promoting adhesion to cellular integrin receptors. The 3-D Life Scrambled RGD Peptide contains a scrambled RGD motif and a thiol group on the N-terminus. The thiol group is used to immobilize the peptide on maleimide polymers in 3-D Life Hydrogels. The immobilized peptide is used in control experiments to determine the specificity of cell adhesion caused by a genuine RGD peptide (e.g. 3-D Life RGD Peptide).
For instructions, please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
3D cell culture: Covalent attachment to thiol-reactive polymer in 3-D Life Hydrogels. For control experiments to determine specificity of cell adhesion to RGD Peptide.
Quantity
Cat. No. 09-P-003: 1 µmol
Cat. No. P11-3: 3x 1 µmol
Components
Cat. No. 09-P-003:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| Scrambled RGD Peptide, lyophilized | 1 µmol | 20 mmol/L* | MSDS | 
| Water | 600 µl | n/a | MSDS | 
| *Volume/concentration after reconstitution of lyophilizate. | |||
Cat. No. P11-3:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| Scrambled RGD Peptide, lyophilized | 3 x 1 µmol | 20 mmol/L* | MSDS | 
| Water | 600 µl | n/a | MSDS | 
| *Volume/concentration after reconstitution of lyophilizate. | |||
Peptide sequence
Acetyl-Cys-Doa*-Doa-Gly-Arg-Asp-Gly-Ser-Pro-NH2
*: Doa:8-amino-3,6-dioxaoctanoic acid
Molar mass
1022 g/mol
Purity
>90% (HPLC)
Resources
- Product Datasheet (PDS): 3-D Life Scrambled RGD Peptide (1 µmol)
 - Product Datasheet (PDS): 3-D Life Scrambled RGD Peptide (3x 1 µmol)
 - 
3-D Life User Guide & Protocols
 - 3-D Life Biomimetic Hydrogels Page
 - 3-D Life Hydrogels User Applications
 - 3-D Life Hydrogels Product References
 
Application Notes
- Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels
 - Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
 
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.
              
            
      
      
      
      


