Description
RGD sequences are motifs on extracellular matrix proteins promoting adhesion to cellular integrin receptors. The 3-D Life RGD Peptide contains a RGD motif and a thiol group on the N-terminus. The thiol group is used to immobilize the peptide on thiol-reactive polymers in 3-D Life Hydrogels. The immobilized peptide promotes adhesion of cells carrying the appropriate integrin receptor.
For instructions, please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
Modification of 3-D Life Hydrogels with cell-adhesive properties. 3-D cell culture of cells requiring a cell-adhesive matrix based on the RGD motif.
Quantity
Cat. No. 09-P-001: 1 µmol
Cat. No. P10-3: 3x 1 µmol
Components
Cat. No. 09-P-001:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| RGD Peptide, lyophilized | 1 µmol | 20 mmol/L* | MSDS |
| Water | 600 µl | n.a. | MSDS |
| *Volume/concentration after reconstitution of lyophilisate. | |||
Cat. No. P10-3:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| RGD Peptide, lyophilized | 3 x 1 µmol | 20 mmol/L* | MSDS |
| Water | 600 µl | n.a. | MSDS |
| *Volume/concentration after reconstitution of lyophilisate. | |||
Peptide sequence
Acetyl-Cys-Doa*-Doa-Gly-Arg-Gly-Asp-Ser-Pro-NH2
*: Doa:8-amino-3,6-dioxaoctanoic acid
Molar mass
1022 g/mol
Purity
>90% (HPLC)
Resources
- Product Datasheet: 3-D Life RGD Peptide (1 µmol)
- Product Datasheet: 3-D Life RGD Peptide (3x 1 µmol)
- Protocol: Preparation of 3-D Life Fast (FG) Gelling Hydrogels
- Protocol: Preparation of 3-D Life Slow Gelling (SG) Hydrogels
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Application Notes
- Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels
- Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels
- Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels
- Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
- Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.