Description
RGD sequences are motifs on extracellular matrix proteins promoting adhesion to cellular integrin receptors. The 3-D Life RGD Peptide contains a RGD motif and a thiol group on the N-terminus. The thiol group is used to immobilize the peptide on thiol-reactive polymers in 3-D Life Hydrogels. The immobilized peptide promotes adhesion of cells carrying the appropriate integrin receptor.
For instructions, please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
Modification of 3-D Life Hydrogels with cell-adhesive properties. 3D cell culture of cells requiring a cell-adhesive matrix based on the RGD motif.
Quantity
Cat. No. 09-P-001: 1 µmol
Cat. No. P10-3: 3x 1 µmol
Components
Cat. No. 09-P-001:
Material | Quantity | Concentration of reactive groups | |
---|---|---|---|
RGD Peptide, lyophilized | 1 µmol | 20 mmol/L* | MSDS |
Water | 600 µl | n.a. | MSDS |
*Volume/concentration after reconstitution of lyophilisate. |
Cat. No. P10-3:
Material | Quantity | Concentration of reactive groups | |
---|---|---|---|
RGD Peptide, lyophilized | 3 x 1 µmol | 20 mmol/L* | MSDS |
Water | 600 µl | n.a. | MSDS |
*Volume/concentration after reconstitution of lyophilisate. |
Peptide sequence
Acetyl-Cys-Doa*-Doa-Gly-Arg-Gly-Asp-Ser-Pro-NH2
*: Doa:8-amino-3,6-dioxaoctanoic acid
Molar mass
1022 g/mol
Purity
>90% (HPLC)
Resources
- Product Datasheet (PDS): 3-D Life RGD Peptide (1 µmol)
- Product Datasheet (PDS): 3-D Life RGD Peptide (3x 1 µmol)
- Protocol: Preparation of 3-D Life Fast (FG) Gelling Hydrogels
- Protocol: Preparation of 3-D Life Slow Gelling (SG) Hydrogels
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Application Notes
- Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels
- Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels
- Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels
- Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
- Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels
Product References
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- Narasimhan, Badri Narayanan, and Stephanie I Fraley. “Degradability tunes ECM stress relaxation and cellular mechanics.” bioRxiv : the preprint server for biology 2024.07.28.605514. 29 Jul. 2024, doi:10.1101/2024.07.28.605514. Preprint.
- Cipriano, Madalena et al. “Human immunocompetent choroid-on-chip: a novel tool for studying ocular effects of biological drugs.” Communications biology vol. 5,1 52. 13 Jan. 2022, doi:10.1038/s42003-021-02977-3
- Kuehlbach, Claudia et al. “Recapitulating the Angiogenic Switch in a Hydrogel-Based 3D In Vitro Tumor-Stroma Model.” Bioengineering (Basel, Switzerland) vol. 8,11 186. 15 Nov. 2021, doi:10.3390/bioengineering8110186
- Sun, Xue et al. “CloneSeq - Single-cell clonal 3D culture and analysis protocol.” STAR protocols vol. 2,4 100794. 29 Sep. 2021, doi:10.1016/j.xpro.2021.100794
- Nair, Arya Lekshmi et al. “Parallelizable Microfluidic Platform to Model and Assess In Vitro Cellular Barriers: Technology and Application to Study the Interaction of 3D Tumor Spheroids with Cellular Barriers.” Biosensors vol. 11,9 314. 3 Sep. 2021, doi:10.3390/bios11090314
- Roy, Tanaya et al. “Anti-VEGF-R2 Aptamer and RGD Peptide Synergize in a Bifunctional Hydrogel for Enhanced Angiogenic Potential.” Macromolecular bioscience vol. 21,2 (2021): e2000337. doi:10.1002/mabi.202000337
- Georgakopoulos, Nikitas et al. “Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.” BMC developmental biology vol. 20,1 4. 26 Feb. 2020, doi:10.1186/s12861-020-0209-5
- Wang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” Materials (Basel, Switzerland) vol. 12,20 3391. 17 Oct. 2019, doi:10.3390/ma12203391
- Friedrich, Oliver et al. “Stretch in Focus: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” Frontiers in bioengineering and biotechnology vol. 7 55. 27 Mar. 2019, doi:10.3389/fbioe.2019.00055
- Grobe, Hanna et al. “A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions.” PloS one vol. 13,3 e0194716. 26 Mar. 2018, doi:10.1371/journal.pone.0194716
- Nugraha, Bramasta et al. “Monitoring and manipulating cellular crosstalk during kidney fibrosis inside a 3D in vitro co-culture.” Scientific reports vol. 7,1 14490. 3 Nov. 2017, doi:10.1038/s41598-017-12683-y
- Grikscheit, Katharina et al. “Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1.” The Journal of cell biology vol. 209,3 (2015): 367-76. doi:10.1083/jcb.201412015
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