Ilex Life Sciences Ultra pure Actin consists in the alpha-skeletal muscle isoform and is purified from rabbit striated muscle. The purification method used (according to Spudich & Watts) results in a highly purified protein with a molecular mass of 43,000 dalton. Small vial.
Ilex Life Sciences Ultra pure Actin consists in the alpha-skeletal muscle isoform and is purified from rabbit striated muscle. The purification method used (according to Spudich & Watts) results in a highly purified protein with a molecular mass of 43,000 dalton. Large vial.
  • Load image into Gallery viewer, Ilex Life Sciences Ultra pure Actin consists in the alpha-skeletal muscle isoform and is purified from rabbit striated muscle. The purification method used (according to Spudich & Watts) results in a highly purified protein with a molecular mass of 43,000 dalton. Small vial.
  • Load image into Gallery viewer, Ilex Life Sciences Ultra pure Actin consists in the alpha-skeletal muscle isoform and is purified from rabbit striated muscle. The purification method used (according to Spudich & Watts) results in a highly purified protein with a molecular mass of 43,000 dalton. Large vial.

Actin, Alpha Skeletal Muscle, Rabbit Protein

A1517050
Regular price
$195.00
Sale price
$195.00
Regular price
$232.00
Temporarily out of stock
Unit price
per 
Shipping calculated at checkout.

Description

Ultra pure Actin in the alpha-skeletal muscle isoform, purified from rabbit striated muscle. The purification method used (according to Spudich & Watts) results in a highly purified protein with a molecular mass of 43,000 dalton.

Summary

Actin is a muscle protein localized in the I band of the myofibrils; acting along with myosin, it is responsible for contraction and relaxation of muscle. Each actin protomer binds one molecule of ATP and has one high affinity site for either calcium or magnesium ions, as well as several low affinity sites. Actin exists as a monomer in low salt concentrations, but filaments form rapidly as salt concentration rises, with the consequent hydrolysis of ATP. It occurs in globular (G-actin) and fibrous (F-actin) forms. Actin is found in all eukaryotic cells (except for nematode sperm). Actin is one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Its other functions include cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.

Source

Rabbit muscle.

Formulation

Sterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized from a 1mg/ml solution containing 10mM Tris/HCl buffer pH 8.0, 0.2mM CaCl2, 0.2mM ATP, 1mM DTT and 0.5% (w/v) SDS.

Purity

Greater than 98.0%.

Reconstitution

It is recommended to reconstitute the lyophilized Actin in sterile 18MΩ-cm H2O not less than 1mg/ml, which can then be further diluted to other aqueous solutions.

Shipping

At ambient temperature.  Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized Actin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Actin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Usage

This product is intended to Laboratory Research Use Only.  This product may not be used a drug, agricultural product, food additives.

Other Names

Alpha-actin-1, ACTA1 gene product, actin alpha isoform, UniProtKB - P68135 (ACTS_RABIT)

Resources

Want to learn more about actin, alpha skeletal muscle isoform? We have compiled the links below which contain information that you may find interesting.